一种用于定量巨噬细胞从琼脂糖微滴迁移的酶联免疫吸附测定法。
An ELISA method for quantitation of macrophage migration from agarose microdroplets.
作者信息
Peck R
出版信息
J Immunol Methods. 1983 Nov 11;64(1-2):179-87. doi: 10.1016/0022-1759(83)90396-4.
PMID:6358361
Abstract
A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus detected correlate well with the migration as measured by a capillary tube assay for MIF.
摘要
描述了一种基于检测从琼脂糖液滴迁移的巨噬细胞来定量巨噬细胞迁移抑制因子（MIF）生物活性的方法。在酶联免疫吸附测定（ELISA）中用多克隆抗巨噬细胞抗体测定迁移情况。如此检测到的巨噬细胞数量与通过MIF毛细管测定法测得的迁移情况密切相关。
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