Schriewer H, Jung G, Emke F, Assmann G
J Clin Chem Clin Biochem. 1983 Oct;21(10):611-4. doi: 10.1515/cclm.1983.21.10.611.
An economical enzymatic assay for HDL phosphatidyl choline is described, as adapted for the Cobas-Bio analyser (Hoffmann LaRoche). This method entails the enzymatic cleavage of phosphatidyl choline by phospholipase C from B. cereus, hydrolysis of phosphoryl choline and enzymatic determination of choline with choline oxidase by an enzymatic colour test. This method provides consistent values and is, by comparison to the enzymatic UV method (assaying choline with choline kinase in an optical test procedure), simpler to perform, more precise, and less expensive.
本文描述了一种适用于Cobas-Bio分析仪（霍夫曼·罗氏公司）的、用于检测高密度脂蛋白磷脂酰胆碱的经济酶法。该方法包括用蜡样芽孢杆菌的磷脂酶C对磷脂酰胆碱进行酶解、磷酸胆碱的水解以及通过酶促比色法用胆碱氧化酶对胆碱进行酶促测定。与酶促紫外法（在光学检测程序中用胆碱激酶检测胆碱）相比，该方法提供的结果一致，操作更简单、更精确且成本更低。
