Schriewer H, Jabs H U, Assmann G
J Clin Chem Clin Biochem. 1982 May;20(5):305-12.
A simple method is described for the enzymatic determination of sphingomyelin in the apolipoprotein B-free supernatants prepared by precipitation of blood sera with phosphotungstate/MgCl2. The analysis is based on the enzymatic hydrolysis of sphingomyelin, by sphingomyelinase from B. cereus, into phosphorylcholine and N-acylsphingosine, and subsequent hydrolysis of phosphorylcholine by alkaline phosphatase. The choline formed is determined by choline kinase in an optical test. The results from this method were in good agreement with those obtained by the conventional chemical sphingomyelin determination. Furthermore, there was a good correlation between the sphingomyelin concentrations obtained from the HDL fractions isolated by ultracentrifugation (1.063-1.21 kg/l) and those obtained from the apolipoprotein B free supernatants after phosphotungstate/mgCl2 precipitation of sera.
描述了一种简单的方法，用于酶法测定用磷钨酸盐/MgCl₂沉淀血清制备的无载脂蛋白B上清液中的鞘磷脂。该分析基于蜡样芽孢杆菌的鞘磷脂酶将鞘磷脂酶促水解为磷酸胆碱和N-酰基鞘氨醇，随后碱性磷酸酶将磷酸胆碱水解。形成的胆碱通过胆碱激酶在光学测试中进行测定。该方法的结果与通过传统化学方法测定鞘磷脂的结果高度一致。此外，通过超速离心（1.063-1.21 kg/l）分离的高密度脂蛋白（HDL）组分中获得的鞘磷脂浓度与血清经磷钨酸盐/MgCl₂沉淀后从无载脂蛋白B上清液中获得的鞘磷脂浓度之间存在良好的相关性。
