Brown T J, Ercolani L, Ginsberg B H
J Recept Res. 1983;3(4):481-94. doi: 10.3109/10799898309041854.
Primary human T lymphocytes that have been mitogen activated in chemically defined medium express cell surface insulin receptors. The receptor is identical to other mammalian insulin receptors in binding properties, including: pH dependency, ligand affinity, hormone specificity, and cooperative interactions. Scatchard plots are curvilinear and a ligand-induced increase in dissociation, the property normally associated with "negative cooperativity", is kinetically demonstrable. In vitro insulin treatment of the receptor-negative, resting T lymphocyte slightly enhances the degree of insulin binding which emerges following cellular activation. Insulin treatment of receptor-positive lymphoblasts results in insulin receptor "down-regulation". These findings indicate that T lymphoblast insulin receptor concentrations are not significantly influenced by insulin before their emergence but are dramatically regulated by insulin following their appearance at the cell surface.
在化学成分明确的培养基中经有丝分裂原激活的原代人T淋巴细胞表达细胞表面胰岛素受体。该受体在结合特性方面与其他哺乳动物胰岛素受体相同，包括：pH依赖性、配体亲和力、激素特异性和协同相互作用。Scatchard图呈曲线状，并且配体诱导的解离增加（通常与“负协同性”相关的特性）在动力学上是可证明的。对受体阴性的静止T淋巴细胞进行体外胰岛素处理，会略微增强细胞激活后出现的胰岛素结合程度。对受体阳性的淋巴母细胞进行胰岛素处理会导致胰岛素受体“下调”。这些发现表明，T淋巴母细胞胰岛素受体浓度在其出现之前不受胰岛素的显著影响，但在其出现在细胞表面之后会受到胰岛素的显著调节。
