视网膜神经胶质细胞向晶状体表型的转化：晶状体质膜抗原MP26的表达。

We describe experiments in which dissociated cells from differentiated, post-mitotic neural retina of late chicken embryos (13 and 16 days) rapidly and consistently transform (transdifferentiate) in vitro into lens-like phenotype and form spherical lentoids. Using immunohistochemical and other tests, we have established that the lentoids arise from the progeny of definitive retinal glia cells (Müller cells). An early event in their transformation is the appearance in the cell surface of MP26, a plasma membrane protein characteristic of lens but not found in the retina. The results support the hypothesis that the phenotype of definitive glia cells in the retina is stabilized by contact-mediated interactions with neurons; disruption of cell contacts and cell separation alter surface properties of the glia cells, decontrol their phenotype, and predispose them to phenotype transformation.

我们描述了这样一些实验

从晚期鸡胚（13天和16天）分化后的有丝分裂后神经视网膜分离出的细胞，在体外迅速且持续地转变（转分化）为晶状体样表型并形成球形类晶状体。通过免疫组织化学和其他检测，我们确定类晶状体源自终末视网膜神经胶质细胞（穆勒细胞）的后代。它们转变过程中的一个早期事件是MP26出现在细胞表面，MP26是一种晶状体特有的质膜蛋白，在视网膜中未发现。这些结果支持了这样的假说：视网膜中终末神经胶质细胞的表型通过与神经元的接触介导相互作用得以稳定；细胞接触的破坏和细胞分离改变了神经胶质细胞的表面特性，解除了对其表型的控制，并使其易于发生表型转变。