大鼠腹水肝癌染色质中H1组蛋白对蛋白水解攻击的抗性

Resistance of H1 histone to proteolytic attack in chromatin from rat-ascites hepatoma.

作者信息

Kishida K, Sugano N

出版信息

Cancer Lett. 1983 Dec;21(2):125-31. doi: 10.1016/0304-3835(83)90198-2.

Abstract

From rat-liver and ascites-hepatoma chromatins, NaCl-soluble fractions were prepared. The 0.35 M NaCl-soluble fraction from the hepatoma (AH) chromatin contained much non-histone protein of high-molecular weight, compared with the fraction from the rat-liver (RL) chromatin. The 0.35 M NaCl-insoluble, but 2 M NaCl/5 M urea-soluble fraction was composed mainly of 5 classes of histones. These histones were quantitatively not different between AH and RL chromatins. However, H1 histone was rather protease-resistant in AH chromatin, but not in RL chromatin. The proteolytic capacity was also lower in AH chromatin. In addition, in the micrococcal-nuclease digest of AH nuclei, the oligonucleosomes were considerably retained even by long-time digestion, but not in that of RL nuclei.

摘要

从大鼠肝脏和腹水肝癌染色质中制备了NaCl可溶性组分。与大鼠肝脏（RL）染色质的组分相比，肝癌（AH）染色质的0.35M NaCl可溶性组分含有许多高分子量的非组蛋白。0.35M NaCl不溶性但2M NaCl/5M尿素可溶性组分主要由5类组蛋白组成。这些组蛋白在AH和RL染色质之间在数量上没有差异。然而，H1组蛋白在AH染色质中对蛋白酶具有相当的抗性，但在RL染色质中则不然。AH染色质中的蛋白水解能力也较低。此外，在AH细胞核的微球菌核酸酶消化中，即使长时间消化，寡核小体也能大量保留，但在RL细胞核中则不然。