组蛋白H1[0]在染色质结构中的作用。

The involvement of histone H1[0] in chromatin structure.

作者信息

Roche J, Girardet J L, Gorka C, Lawrence J J

出版信息

Nucleic Acids Res. 1985 Apr 25;13(8):2843-53. doi: 10.1093/nar/13.8.2843.

DOI:10.1093/nar/13.8.2843

PMID:4000966

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341198/

Abstract

Micrococcal nuclease digestion and light scattering are used to compare native chromatins with various histone H1[0] contents. The experimental data show that the higher the H1[0] content, the greater the ability to form compact structures with increasing ionic strength, and the lower the DNA accessibility to micrococcal nuclease. On the contrary, reconstituted samples from H1-depleted chromatin and pure individual H1 fractions behave in such a way that samples reconstituted with pure H1 degree give rise to a looser structure, more accessible to nuclease than samples reconstituted with H1-1. This contradiction suggests that the effect of H1o on chromatin structure must originate from the interaction of this histone with other components in native chromatin among which other histone H1 subfractions are good candidates.

摘要

利用微球菌核酸酶消化和光散射来比较具有不同组蛋白H1[0]含量的天然染色质。实验数据表明，H1[0]含量越高，随着离子强度增加形成紧密结构的能力越强，且微球菌核酸酶对DNA的可及性越低。相反，来自H1缺失染色质和纯单个H1组分的重组样品表现出，用纯H1程度重组的样品产生的结构更松散，与用H1-1重组的样品相比，对核酸酶更易接近。这种矛盾表明，H1o对染色质结构的影响必定源于该组蛋白与天然染色质中其他组分的相互作用，其中其他组蛋白H1亚组分是很好的候选者。