Blaszczyk M, Pak K Y, Herlyn M, Lindgren J, Pessano S, Steplewski Z, Koprowski H
Cancer Res. 1984 Jan;44(1):245-53.
Four major carcinoembryonic antigen-related glycopeptides (Mr 180,000, 160,000, 50,000, and 40,000) were detected in SW948 colon carcinoma cells and in colon adenocarcinoma tissue using a monoclonal antibody (C(4)20-32) generated by immunizing mice with SW1222 human colon carcinoma cells. Only the Mr 50,000 polypeptide was immunoprecipitated from normal colon mucosa by this antibody. Binding studies using other monoclonal antibodies and lectins indicated the different epitopes and carbohydrate attachment sites on each of the four polypeptides. Only monoclonal antibody C(4)20-32 recognized a common determinant on all four polypeptides which was revealed by its reactivity with each affinity-purified component.
使用通过用SW1222人结肠癌细胞免疫小鼠产生的单克隆抗体（C(4)20 - 32），在SW948结肠癌细胞和结肠腺癌组织中检测到四种主要的癌胚抗原相关糖肽（分子量分别为180,000、160,000、50,000和40,000）。该抗体仅从正常结肠黏膜中免疫沉淀出分子量为50,000的多肽。使用其他单克隆抗体和凝集素进行的结合研究表明，这四种多肽各自具有不同的表位和碳水化合物附着位点。只有单克隆抗体C(4)20 - 32识别所有四种多肽上的一个共同决定簇，这通过其与每种亲和纯化成分的反应性得以揭示。
