Falus A, Rasmussen J M, Svehag S E
Immunol Lett. 1983;7(2):61-4. doi: 10.1016/0165-2478(83)90034-2.
beta 2-microglobulin (beta 2m)-containing, rapidly sedimenting peaks were seen when sera of patients with 3% polyethylene glycol (PEG)-insoluble beta 2m were fractionated by sucrose density gradient ultracentrifugation. No such material was found in serum of a healthy volunteer without 3% PEG-insoluble beta 2m. MW of beta 2m-containing high molecular weight fractions ranged from 1.6 X 10(5) to 2.1 X 10(6). When some fractions were recentrifuged a majority of beta 2m was recovered at the original sedimentation position. Analysis of high molecular weight beta 2m-containing peaks by SDS-PAGE followed by electroblotting revealed only monomeric beta 2m indicating that beta 2m was not covalently bound in the high molecular weight material.
当用蔗糖密度梯度超速离心法对含有3%聚乙二醇（PEG）不溶性β2微球蛋白（β2m）的患者血清进行分级分离时，观察到含有β2m的快速沉降峰。在没有3% PEG不溶性β2m的健康志愿者血清中未发现此类物质。含有β2m的高分子量组分的分子量范围为1.6×10⁵至2.1×10⁶。当对一些组分进行再次离心时，大部分β2m在原始沉降位置被回收。通过SDS-PAGE随后进行电印迹对含有高分子量β2m的峰进行分析，结果仅显示单体β2m，这表明β2m在高分子量物质中不是共价结合的。
