Watanabe H, Timmis K N
Infect Immun. 1984 Jan;43(1):391-6. doi: 10.1128/iai.43.1.391-396.1984.
The role of plasmids in the virulence of Shigella dysenteriae 1 W30864, which contains at least five species, was investigated. By means of a standard plasmid-curing procedure, that is, bacterial cultivation at an elevated temperature, five virulence-deficient derivatives were obtained. One of these lacked a small, 6-megadalton plasmid, designated pHW400, exhibited reduced invasiveness for HeLa cells, and failed to produce the somatic antigen. Transposon tagging of the pHW400 plasmid to produce pHW401 and the transfer of this derivative into a variant of strain W30864 lacking pHW400 confirmed the conclusion that the pHW400 plasmid encodes one or more functions involved in O antigen (lipopolysaccharide) biosynthesis and bacterial virulence. A plasmid of similar size was detected in all of the three other strains of S. dysenteriae 1 examined.
研究了质粒在痢疾志贺氏菌1型W30864（其至少包含五个种）毒力中的作用。通过标准的质粒消除程序，即细菌在高温下培养，获得了五种毒力缺陷衍生物。其中之一缺少一个6兆道尔顿的小质粒，命名为pHW400，对HeLa细胞的侵袭性降低，且无法产生菌体抗原。对pHW400质粒进行转座子标签标记以产生pHW401，并将该衍生物转移到缺乏pHW400的W30864菌株变体中，证实了pHW400质粒编码参与O抗原（脂多糖）生物合成和细菌毒力的一种或多种功能这一结论。在所检测的其他三株痢疾志贺氏菌1型中均检测到了类似大小的质粒。
