酿酒酵母基于2微米环状的人工质粒的拷贝数与稳定性
Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.
作者信息
Futcher A B, Cox B S
出版信息
J Bacteriol. 1984 Jan;157(1):283-90. doi: 10.1128/jb.157.1.283-290.1984.
Abstract
The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, high copy number is associated with low instability; (iii) it is difficult to reconcile this variability with a strict and direct system of copy number control; (iv) instabilities are much higher than expected from random partition and the observed copy numbers: this may imply partition which is less efficient than random. Even so, (v) the partitioning of 2-micron circle-like plasmids is more efficient than that of ARS-based plasmids, which hints at the existence of a system for the (inefficient) distribution of 2-micron circles.
摘要
已在酿酒酵母的[Cir+]和[Cir0]菌株中准确测量了基于人工2微米环状质粒的拷贝数和稳定性。我们得出以下结论：（i）不同质粒的不稳定性和拷贝数差异很大；（ii）不稳定性和拷贝数呈负相关——也就是说，高拷贝数与低不稳定性相关；（iii）难以将这种变异性与严格且直接的拷贝数控制系统相协调；（iv）不稳定性远高于随机分配和观察到的拷贝数所预期的水平：这可能意味着分配效率低于随机分配。即便如此，（v）2微米环状样质粒的分配比基于自主复制序列（ARS）的质粒更有效，这暗示存在一个用于（低效）分配2微米环的系统。
相似文献
1
Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.
J Bacteriol. 1984 Jan;157(1):283-90. doi: 10.1128/jb.157.1.283-290.1984.
2
Reconstruction of the yeast 2 micron plasmid partitioning mechanism.
Nucleic Acids Res. 1988 Jul 25;16(14B):7103-17. doi: 10.1093/nar/16.14.7103.
3
2-micron circle plasmids do not reduce yeast life span.
FEMS Microbiol Lett. 2005 Sep 15;250(2):245-51. doi: 10.1016/j.femsle.2005.07.018.
4
Copy number and partition of the Saccharomyces cerevisiae 2 micron plasmid controlled by transcription regulators.
Mol Cell Biol. 1988 Nov;8(11):4949-57. doi: 10.1128/mcb.8.11.4949-4957.1988.
5
The 2 micron plasmid of Saccharomyces cerevisiae: a miniaturized selfish genome with optimized functional competence.
Plasmid. 2013 Jul;70(1):2-17. doi: 10.1016/j.plasmid.2013.03.001. Epub 2013 Mar 27.
6
The partitioning and copy number control systems of the selfish yeast plasmid: an optimized molecular design for stable persistence in host cells.
Microbiol Spectr. 2014 Oct;2(5). doi: 10.1128/microbiolspec.PLAS-0003-2013.
7
Copy number amplification of the 2 micron circle plasmid of Saccharomyces cerevisiae.
J Theor Biol. 1986 Mar 21;119(2):197-204. doi: 10.1016/s0022-5193(86)80074-1.
8
Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.
Curr Genet. 1986;11(1):79-82. doi: 10.1007/BF00389429.
9
[Isolation of recipient yeast strains for the stable reproduction of 2-micron DNA vectors].
Mol Biol (Mosk). 1986 Sep-Oct;20(5):1273-80.
10
Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae.
J Bacteriol. 1979 Oct;140(1):73-82. doi: 10.1128/jb.140.1.73-82.1979.
引用本文的文献
1
Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies.
Sci Rep. 2025 May 26;15(1):18368. doi: 10.1038/s41598-025-02380-6.
2
A versatile genetic toolkit for engineering for tetraacetyl phytosphingosine production.
Front Bioeng Biotechnol. 2025 Apr 28;13:1586218. doi: 10.3389/fbioe.2025.1586218. eCollection 2025.
3
Cross-feeding promotes heterogeneity within yeast cell populations.
Nat Commun. 2024 Jan 10;15(1):418. doi: 10.1038/s41467-023-44623-y.
4
A Tunable and Expandable Transactivation System in Probiotic Yeast .
ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23.
5
Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.
G3 (Bethesda). 2021 Dec 8;11(12). doi: 10.1093/g3journal/jkab359.
6
Synthetic production of prenylated naringenins in yeast using promiscuous microbial prenyltransferases.
Metab Eng Commun. 2021 Mar 19;12:e00169. doi: 10.1016/j.mec.2021.e00169. eCollection 2021 Jun.
7
Structure-based directed evolution improves growth on xylose by influencing in vivo enzyme performance.
Biotechnol Biofuels. 2020 Jan 11;13:5. doi: 10.1186/s13068-019-1643-0. eCollection 2020.
8
Optimal parameter identification of synthetic gene networks using harmony search algorithm.
PLoS One. 2019 Mar 29;14(3):e0213977. doi: 10.1371/journal.pone.0213977. eCollection 2019.
9
Insights into the DNA sequence elements required for partitioning and copy number control of the yeast 2-micron plasmid.
Curr Genet. 2019 Aug;65(4):887-892. doi: 10.1007/s00294-019-00958-y. Epub 2019 Mar 26.
10
Utilization of a Strongly Inducible Promoter to Control Gene Expression in .
Front Microbiol. 2018 Nov 16;9:2736. doi: 10.3389/fmicb.2018.02736. eCollection 2018.
本文引用的文献
1
Replication and recombination functions associated with the yeast plasmid, 2 mu circle.
Cell. 1980 Sep;21(2):501-8. doi: 10.1016/0092-8674(80)90487-0.
2
Properties of a Saccharomyces cerevisiae mtDNA segment conferring high-frequency yeast transformation.
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1578-82. doi: 10.1073/pnas.79.5.1578.
3
Characterization of the transmission during cytoductant formation of the 2 micrometers DNA plasmid from Saccharomyces.
Mol Gen Genet. 1981;183(1):59-65. doi: 10.1007/BF00270139.
4
Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.
J Bacteriol. 1981 Mar;145(3):1421-4. doi: 10.1128/jb.145.3.1421-1424.1981.
5
Maintenance of the 2 microns circle plasmid in populations of Saccharomyces cerevisiae.
J Bacteriol. 1983 May;154(2):612-22. doi: 10.1128/jb.154.2.612-622.1983.
6
Sequence variation in the LEU2 region of the saccharomyces cerevisiae genome.
Gene. 1981 Dec;16(1-3):133-9. doi: 10.1016/0378-1119(81)90069-x.
7
A stable plasmid carrying the yeast Leu2 gene and containing only yeast deoxyribonucleic acid.
J Bacteriol. 1980 Jan;141(1):413-6. doi: 10.1128/jb.141.1.413-416.1980.
8
The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae.
J Mol Biol. 1969 Mar 14;40(2):261-77. doi: 10.1016/0022-2836(69)90474-4.
9
Isolation of yeast DNA.
Methods Cell Biol. 1975;12:39-44. doi: 10.1016/s0091-679x(08)60950-4.
10
Inheritability of plasmids and population dynamics of cultured cells.
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4085-9. doi: 10.1073/pnas.72.10.4085.
Zotero 插件