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从酿酒酵母中去除2微米DNA质粒

Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.

作者信息

Toh-e A, Wickner R B

出版信息

J Bacteriol. 1981 Mar;145(3):1421-4. doi: 10.1128/jb.145.3.1421-1424.1981.

DOI:10.1128/jb.145.3.1421-1424.1981
PMID:7009587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217149/
Abstract

The 2 mu DNA plasmid is often eliminated from yeast cells when they are transformed with the 2 mu DNA-LEU2-pMB9 composite plasmid pJDB219. Since pJDB219 is subsequently lost with high frequency, derivatives lacking all 2 mu DNA can be prepared from any strain.

摘要

当用2μm DNA-LEU2-pMB9复合质粒pJDB219转化酵母细胞时，2μm DNA质粒常常会从酵母细胞中消除。由于pJDB219随后会高频丢失，因此可以从任何菌株中制备出不含所有2μm DNA的衍生物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fcd/217149/dc36f087ecd3/jbacter00274-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fcd/217149/d8d66f9c388f/jbacter00274-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fcd/217149/dc36f087ecd3/jbacter00274-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fcd/217149/d8d66f9c388f/jbacter00274-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fcd/217149/dc36f087ecd3/jbacter00274-0311-a.jpg

相似文献

1
Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.从酿酒酵母中去除2微米DNA质粒
J Bacteriol. 1981 Mar;145(3):1421-4. doi: 10.1128/jb.145.3.1421-1424.1981.
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A stable plasmid carrying the yeast Leu2 gene and containing only yeast deoxyribonucleic acid.一种携带酵母亮氨酸2基因且仅包含酵母脱氧核糖核酸的稳定质粒。
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The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.酿酒酵母2微米DNA重组质粒上存在有缺陷的LEU2基因，这导致了质粒的消除和高拷贝数。
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Introduction of nonselectible 2 mu plasmid into [cir(o)] cells of the yeast S. cerevisiae by DNA transformation and in vivo site-specific resolution.通过DNA转化和体内位点特异性切割将非选择性2μm质粒导入酿酒酵母的[cir(o)]细胞。
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Autonomously replicating sequences in Saccharomyces cerevisiae.酿酒酵母中的自主复制序列。
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[Isolation of recipient yeast strains for the stable reproduction of 2-micron DNA vectors].[用于2微米DNA载体稳定复制的受体酵母菌株的分离]
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引用本文的文献

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The effect of canavanine on the maintenance in yeast of chimeric plasmids containing portions of the 2-µm DNA plasmid.刀豆氨酸对含 2μmDNA 质粒部分片段的嵌合质粒在酵母中维持的影响。
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本文引用的文献

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Mitochondrial-satellite and circular DNA filaments in yeast.酵母中的线粒体卫星和环状DNA细丝。
Science. 1967 Jun 2;156(3779):1234-7. doi: 10.1126/science.156.3779.1234.
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[Gamma satellite DNA and small twisted circular molecules in yeast Saccharomyces cerevisiae].[酿酒酵母中的γ卫星DNA和小的扭曲环状分子]
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Replication of a bacterial episome under relaxed control.在松弛控制下细菌附加体的复制。
在酿酒酵母中显示出非孟德尔遗传和热诱导性的两个新的双链RNA分子。
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Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.酿酒酵母基于2微米环状的人工质粒的拷贝数与稳定性
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Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT2 gene.酿酒酵母中脯氨酸的利用：克隆的PUT2基因分析
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Introduction of nonselectible 2 mu plasmid into [cir(o)] cells of the yeast S. cerevisiae by DNA transformation and in vivo site-specific resolution.通过DNA转化和体内位点特异性切割将非选择性2μm质粒导入酿酒酵母的[cir(o)]细胞。
Curr Genet. 1989 Feb;15(2):83-90. doi: 10.1007/BF00435453.
7
Self-regulation of 70-kilodalton heat shock proteins in Saccharomyces cerevisiae.酿酒酵母中70千道尔顿热休克蛋白的自我调节
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4
Apparent dispersive replication of yeast mitochondrial DNA as revealed by density labelling experiments.密度标记实验揭示的酵母线粒体DNA的明显分散复制
Mol Gen Genet. 1974;131(3):193-207. doi: 10.1007/BF00267959.
5
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
6
Replicating circular DNA molecules in yeast.在酵母中复制环状DNA分子。
Cell. 1975 Mar;4(3):249-53. doi: 10.1016/0092-8674(75)90172-5.
7
A physical map of the DNA regions flanking the rabbit beta-globin gene.兔β-珠蛋白基因侧翼DNA区域的物理图谱。
Cell. 1977 Oct;12(2):429-39. doi: 10.1016/0092-8674(77)90119-2.
8
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.通过用DNA聚合酶I进行切口平移在体外将脱氧核糖核酸标记至高比活性。
J Mol Biol. 1977 Jun 15;113(1):237-51. doi: 10.1016/0022-2836(77)90052-3.
9
A mutant of Saccharomyces cerevisiae defective for nuclear fusion.一种在细胞核融合方面存在缺陷的酿酒酵母突变体。
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3651-5. doi: 10.1073/pnas.73.10.3651.
10
Circular DNA of a yeast episome with two inverted repeats: structural analysis by a restriction enzyme and electron microscopy.带有两个反向重复序列的酵母附加体环状DNA：用限制性内切酶和电子显微镜进行结构分析
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3030-4. doi: 10.1073/pnas.73.9.3030.