从酿酒酵母中去除2微米DNA质粒
Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.
作者信息
Toh-e A, Wickner R B
出版信息
J Bacteriol. 1981 Mar;145(3):1421-4. doi: 10.1128/jb.145.3.1421-1424.1981.
Abstract
The 2 mu DNA plasmid is often eliminated from yeast cells when they are transformed with the 2 mu DNA-LEU2-pMB9 composite plasmid pJDB219. Since pJDB219 is subsequently lost with high frequency, derivatives lacking all 2 mu DNA can be prepared from any strain.
摘要
当用2μm DNA-LEU2-pMB9复合质粒pJDB219转化酵母细胞时，2μm DNA质粒常常会从酵母细胞中消除。由于pJDB219随后会高频丢失，因此可以从任何菌株中制备出不含所有2μm DNA的衍生物。
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