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用与3微米环状DNA共纯化的DNA将ψ型酿酒酵母转化为ψ⁺型。

Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.

作者信息

Dai H, Tsay S H, Lund P M, Cox B S

机构信息

Institute of Botany, Academia Sinica, Taipei, Republic of China.

出版信息

Curr Genet. 1986;11(1):79-82. doi: 10.1007/BF00389429.

DOI:10.1007/BF00389429
PMID:3329046
Abstract

DNA enriched for supercoiled plasmids prepared from the 3 micron plasmid-enriched, [psi+], [2 micron degrees] strain 6-1G-P188 and from the [2 micron+] [psi+] strain LL20 can be used to transform a psi- recipient strain to psi+. Fractionation of the former preparation by electrophoresis showed that the 3 micron plasmid band contained the transforming activity.

摘要

从富含3微米质粒的[psi+]、[2微米°]菌株6-1G-P188以及[2微米+][psi+]菌株LL20制备的富含超螺旋质粒的DNA，可用于将psi-受体菌株转化为psi+。对前一种制备物进行电泳分级分离表明，3微米质粒条带含有转化活性。

相似文献

1
Transformation of psi- Saccharomyces cerevisiae to psi+ with DNA co-purified with 3 micron circles.用与3微米环状DNA共纯化的DNA将ψ型酿酒酵母转化为ψ⁺型。
Curr Genet. 1986;11(1):79-82. doi: 10.1007/BF00389429.
2
Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.酿酒酵母基于2微米环状的人工质粒的拷贝数与稳定性
J Bacteriol. 1984 Jan;157(1):283-90. doi: 10.1128/jb.157.1.283-290.1984.
3
FLP site-specific recombinase of yeast 2-micron plasmid. Topological features of the reaction.酵母2微米质粒的FLP位点特异性重组酶。反应的拓扑学特征。
J Mol Biol. 1986 Apr 20;188(4):529-44. doi: 10.1016/s0022-2836(86)80003-1.
4
A Dictyostelium discoideum DNA fragment complements a Saccharomyces cerevisiae ura3 mutant.一种盘基网柄菌的DNA片段可互补酿酒酵母ura3突变体。
Gene. 1982 Dec;20(3):433-39. doi: 10.1016/0378-1119(82)90212-8.
5
Stable yeast transformation with chimeric plasmids using a 2 micron-circular DNA-less strain as a recipient.以无2微米环状DNA的菌株作为受体，用嵌合质粒进行稳定的酵母转化。
Mol Gen Genet. 1979 Nov;176(3):335-42. doi: 10.1007/BF00333095.
6
Selective in vitro transcription by purified yeast RNA polymerase II on cloned 2 micron DNA.纯化的酵母RNA聚合酶II对克隆的2μm DNA进行的体外选择性转录。
Nucleic Acids Res. 1981 Aug 25;9(16):3959-78. doi: 10.1093/nar/9.16.3959.
7
3 micron DNA - an extrachromosomal ribosomal DNA in the yeast Saccharomyces cerevisiae.3微米DNA——酿酒酵母中的一种染色体外核糖体DNA。
Gene. 1980 Dec;12(1-2):41-9. doi: 10.1016/0378-1119(80)90014-1.
8
High frequency of yeast transformation by plasmids carrying part or entire 2-micron yeast plasmid.携带部分或完整2微米酵母质粒的质粒对酵母进行转化的频率很高。
Gene. 1979 Mar;5(3):233-53. doi: 10.1016/0378-1119(79)90080-5.
9
[Isolation of recipient yeast strains for the stable reproduction of 2-micron DNA vectors].[用于2微米DNA载体稳定复制的受体酵母菌株的分离]
Mol Biol (Mosk). 1986 Sep-Oct;20(5):1273-80.
10
Yeast plasmids have fewer superhelical turns than predicted by the nucleosome repeat of yeast DNA.酵母质粒的超螺旋圈数比根据酵母DNA核小体重复序列预测的要少。
Biochem Cell Biol. 1991 Feb-Mar;69(2-3):170-7. doi: 10.1139/o91-025.

引用本文的文献

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Int J Mol Sci. 2020 Sep 1;21(17):6340. doi: 10.3390/ijms21176340.
2
[PSI(+)] turns 50.[PSI(+)] 五十岁了。
Prion. 2015;9(5):318-32. doi: 10.1080/19336896.2015.1111508.
3
The genetic control of the formation and propagation of the [PSI+] prion of yeast.酵母[PSI+]朊病毒形成与传播的遗传控制。

本文引用的文献

1
Agents that cause a high frequency of genetic change from [psi+] to [psi-] in Saccharomyces cerevisiae.在酿酒酵母中导致从[psi+]到[psi-]高频遗传变化的因子。
Genetics. 1981 Aug;98(4):691-711. doi: 10.1093/genetics/98.4.691.
2
Reversion analysis of [psi-] mutations in Saccharomyces cerevisiae.
Genet Res. 1981 Apr;37(2):173-82. doi: 10.1017/s0016672300020140.
3
Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs of Saccharomyces cerevisiae and Torulopsis glabrata.酿酒酵母和光滑球拟酵母的细胞质核糖体RNA与3微米环状DNA之间的可杂交序列。
Prion. 2007 Apr-Jun;1(2):101-9. doi: 10.4161/pri.1.2.4665. Epub 2007 Apr 28.
4
Protein folding activity of ribosomal RNA is a selective target of two unrelated antiprion drugs.核糖体RNA的蛋白质折叠活性是两种不相关的抗朊病毒药物的选择性靶点。
PLoS One. 2008 May 14;3(5):e2174. doi: 10.1371/journal.pone.0002174.
5
Multicopy SUP35 gene induces de-novo appearance of psi-like factors in the yeast Saccharomyces cerevisiae.多拷贝SUP35基因诱导酿酒酵母中类似ψ因子的从头出现。
Curr Genet. 1993 Sep;24(3):268-70. doi: 10.1007/BF00351802.
6
Expression and inheritance of the yeast extrachromosomal element psi do not depend on RNA polymerase I.
Curr Genet. 1994 Jan;25(1):49-51. doi: 10.1007/BF00712967.
7
Interactions between chromosomal omnipotent suppressors and extrachromosomal effectors in Saccharomyces cerevisiae.酿酒酵母中染色体全能抑制因子与染色体外效应因子之间的相互作用。
Curr Genet. 1991 Apr;19(4):243-8. doi: 10.1007/BF00355049.
Nucleic Acids Res. 1980 Mar 11;8(5):1009-22. doi: 10.1093/nar/8.5.1009.
4
Relationship of the [psi] factor with other plasmids of Saccharomyces cerevisiae.
Plasmid. 1982 Sep;8(2):103-11. doi: 10.1016/0147-619x(82)90048-8.
5
Structure and function of the nontranscribed spacer regions of yeast rDNA.酵母核糖体DNA非转录间隔区的结构与功能
Nucleic Acids Res. 1984 Mar 26;12(6):2955-68. doi: 10.1093/nar/12.6.2955.
6
3 micron DNA - an extrachromosomal ribosomal DNA in the yeast Saccharomyces cerevisiae.3微米DNA——酿酒酵母中的一种染色体外核糖体DNA。
Gene. 1980 Dec;12(1-2):41-9. doi: 10.1016/0378-1119(80)90014-1.
7
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
8
A mutant of Saccharomyces cerevisiae defective for nuclear fusion.一种在细胞核融合方面存在缺陷的酿酒酵母突变体。
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3651-5. doi: 10.1073/pnas.73.10.3651.
9
Insertion of a genetic marker into the ribosomal DNA of yeast.将遗传标记插入酵母的核糖体DNA中。
Plasmid. 1979 Oct;2(4):536-54. doi: 10.1016/0147-619x(79)90053-2.
10
High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.通过含有克隆的酵母ARG4基因的质粒对酵母进行高频转化。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3829-33. doi: 10.1073/pnas.76.8.3829.