Pichon M F, Spyratos F, Milgrom E
Pathol Biol (Paris). 1983 Nov;31(9):741-5.
Many technical problems are encountered when measuring estradiol and progesterone receptors in breast tumors biopsies. They are due to both intrinsic characteristics of the tissue, and to receptors instability. Suggestions are made to establish an internal quality control in each laboratory at the different steps: obtention and storage of biopsies, purity checking of the reagents, control of the assay protocol, and calculations. Several methods to prepare control pools have been proposed. They use either mammary tumors or animal target organs, and stable concentrations of receptors have been obtained by storage in liquid nitrogen or lyophilization. Published studies have demonstrated important interlaboratory variations. These discrepancies might be in part reduced by using the same assay protocol in the different participating laboratories.
在对乳腺肿瘤活检样本进行雌二醇和孕酮受体检测时会遇到许多技术问题。这些问题既源于组织的固有特性，也源于受体的不稳定性。建议在每个实验室的不同步骤建立内部质量控制：活检样本的获取与保存、试剂纯度检查、检测方案的控制以及计算。已经提出了几种制备对照样本库的方法。这些方法要么使用乳腺肿瘤，要么使用动物靶器官，并且通过液氮保存或冻干获得了稳定的受体浓度。已发表的研究表明不同实验室之间存在显著差异。通过在不同参与实验室使用相同的检测方案，这些差异可能会部分减小。
