Suzuki S, Endo Y, Tanaka S, Iizuka R
Am J Obstet Gynecol. 1984 Jan 1;148(1):76-85. doi: 10.1016/s0002-9378(84)80036-8.
Steroidogenesis in the unfertilized hamster ova was investigated by indirect immunofluorescence study. delta 5-3 beta-Hydroxysteroid dehydrogenase (delta 5-3 beta-HSD) activity was found in hamster follicular and ovulatory ova with pregnenolone used as the substrate. A significant quantity of 17 beta-estradiol was detected in the ooplasm of follicular and ovulatory ova. No progesterone was found. The activity of delta 5-3 beta-HSD in follicular and ovulatory ova was inhibited by preincubation with 10 IU of human chorionic gonadotropin (hCG) in 1 ml of phosphate buffer solution. Trilostane, a potent new inhibitor of the delta 5-3 beta-HSD system, was found to inhibit the activity of delta 5-3 beta-HSD in ovulatory ova in 1 ml of phosphate buffer solution at a concentration of 10(-7)M. After these results were obtained, steroidogenesis of hamster ova was suggested in an indirect immunofluorescence study, and the implications of steroidogenesis for oocyte maturation and subsequent fertilization are discussed.
通过间接免疫荧光研究对未受精仓鼠卵中的类固醇生成进行了调查。以孕烯醇酮为底物时，在仓鼠卵泡期和排卵期的卵中发现了δ5-3β-羟基类固醇脱氢酶（δ5-3β-HSD）活性。在卵泡期和排卵期卵的卵质中检测到大量的17β-雌二醇。未发现孕酮。在1ml磷酸盐缓冲溶液中与10IU人绒毛膜促性腺激素（hCG）预孵育可抑制卵泡期和排卵期卵中δ5-3β-HSD的活性。发现曲洛司坦，一种δ5-3β-HSD系统的强效新型抑制剂，在1ml磷酸盐缓冲溶液中以10(-7)M的浓度可抑制排卵期卵中δ5-3β-HSD的活性。获得这些结果后，在间接免疫荧光研究中提示了仓鼠卵的类固醇生成，并讨论了类固醇生成对卵母细胞成熟和随后受精的影响。
