Suzuki S, Endo Y, Miura R, Iizuka R
Experientia. 1984 Nov 15;40(11):1214-7. doi: 10.1007/BF01946649.
The effects of the inhibition of steroidogenesis by trilostane on oocyte maturation were examined by studying spontaneous maturation and fertilization in vitro. 10(-6)M trilostane had no influence on the meiotic process, whether the oocytes were naked or not. At a concentration of 10(-6)M and 10(-7)M trilostane, low normal pronuclear formation and high polyspermy were found during in vitro fertilization. However, no retarded male pronuclear development could be detected in the trilostane-treated group. Thus, steroid producing activity within ova is apparently necessary to prevent multiple sperm penetration, but it has no effect on meiosis or the action of the so-called male pronucleus growth factor (MPGF).
通过研究体外自发成熟和受精情况，检测了曲洛司坦抑制类固醇生成对卵母细胞成熟的影响。无论卵母细胞有无透明带，10⁻⁶M曲洛司坦对减数分裂过程均无影响。在体外受精过程中，当曲洛司坦浓度为10⁻⁶M和10⁻⁷M时，原核形成正常但多精受精率较高。然而，在曲洛司坦处理组未检测到雄原核发育延迟。因此，卵子内产生类固醇的活性显然是防止多精穿透所必需的，但对减数分裂或所谓的雄原核生长因子（MPGF）的作用没有影响。
