Gupta G S, Singh G
Am J Reprod Immunol (1980). 1983 Oct-Nov;4(3):122-6. doi: 10.1111/j.1600-0897.1983.tb00265.x.
Beta-glucuronidase (beta-glucuronidase) in human seminal plasma consists of two forms of isozymes (major and minor forms). Antiserum prepared against the purified major form of beta-glucuronidase in rabbits was used for studies of immunological specificity and immunohistochemical localization of beta-glucuronidase in the human male reproductive tract. By ouchterlony immunodiffusion technique, anti-beta-glucuronidase serum showed reaction of identity with human tissue extracts such as liver, spleen, lungs, testis, epididymis, prostate, and seminal vesicles. Though monkey testis and epididymis extracts completely reacted with human seminal beta-glucuronidase antiserum, no cross-reaction was observed with mouse and rat tissue extracts. Immunofluorescent studies revealed that beta-glucuronidase is localized in the early stages of spermatogenesis in testis, in columnar cells of the epididymis, and in glandular cells of the prostate and seminal vesicles. Consistent absence of fluorescence in the lumens of testis and epididymis as well as negative cross-reaction between antiserum and sperm extracts suggested that major form of beta-glucuronidase in human seminal plasma is not immunologically identical with human spermatozoa. It is concluded that major form of beta-glucuronidase in seminal plasma is not tissue-specific although it appears to have limited species specificity.
人精浆中的β-葡萄糖醛酸酶由两种同工酶形式（主要形式和次要形式）组成。用兔抗纯化的β-葡萄糖醛酸酶主要形式制备的抗血清，用于研究β-葡萄糖醛酸酶在男性生殖道中的免疫特异性和免疫组织化学定位。通过双向免疫扩散技术，抗β-葡萄糖醛酸酶血清与人组织提取物如肝脏、脾脏、肺、睾丸、附睾、前列腺和精囊呈现同一性反应。虽然猴睾丸和附睾提取物与人精浆β-葡萄糖醛酸酶抗血清完全反应，但未观察到与小鼠和大鼠组织提取物的交叉反应。免疫荧光研究表明，β-葡萄糖醛酸酶定位于睾丸精子发生的早期阶段、附睾的柱状细胞以及前列腺和精囊的腺细胞中。睾丸和附睾管腔中始终没有荧光，以及抗血清与精子提取物之间的阴性交叉反应表明，人精浆中β-葡萄糖醛酸酶的主要形式与人类精子在免疫上不相同。得出的结论是，精浆中β-葡萄糖醛酸酶的主要形式虽然似乎具有有限的物种特异性，但并非组织特异性。
