Sato A, Fleischer A E, Cory J G
Anal Biochem. 1983 Dec;135(2):431-5. doi: 10.1016/0003-2697(83)90706-6.
Deoxyribonucleosides were separated from ribonucleosides by chromatography on polyethyleneimine cellulose columns (Pasteur pipettes. The deoxyribonucleosides were quantitatively eluted with 25 mM boric acid in less than 10 ml while the ribonucleosides were retained. The ribonucleosides were eluted with 1 M NaCl. This method was utilized to assay for GDP, UDP, ADP, and CDP reductase activities after hydrolysis of the substrate and product nucleotides to the corresponding nucleosides. All four reductase activities were assayed using identical conditions of column size, eluting solution (25 mM boric acid), and elution volume. The use of polyethyleneimine cellulose columns with boric acid can be adapted to other enzyme assays such as purine nucleoside phosphorylase and for the isolation of deoxyribonucleotides from cellular extracts.
通过在聚乙烯亚胺纤维素柱（巴斯德吸管）上进行色谱分离，将脱氧核糖核苷与核糖核苷分开。脱氧核糖核苷用25 mM硼酸在不到10 ml的体积中定量洗脱，而核糖核苷则被保留。核糖核苷用1 M NaCl洗脱。该方法用于在将底物和产物核苷酸水解为相应核苷后，测定GDP、UDP、ADP和CDP还原酶的活性。所有四种还原酶活性均在相同的柱尺寸、洗脱液（25 mM硼酸）和洗脱体积条件下进行测定。使用含硼酸的聚乙烯亚胺纤维素柱可适用于其他酶分析，如嘌呤核苷磷酸化酶，以及从细胞提取物中分离脱氧核糖核苷酸。
