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Application of polyethyleneimine cellulose for the class separation of steroidal carboxylic acids from neutral steroids and pigments in urine.

作者信息

Monder C, Iohan F

出版信息

Anal Biochem. 1984 May 15;139(1):237-42. doi: 10.1016/0003-2697(84)90411-1.

DOI:10.1016/0003-2697(84)90411-1
PMID:6742431
Abstract

Metabolites of corticosteroids that contain the 21-oic acid moiety are found in human urine. The acids from neutral steroids and urinary pigments have been separated by passing the mixture through a column of polyethyleneimine cellulose. The acids adhering to the column are quantitatively eluted with dilute formic acid. The purified preparation is suitable for derivatization and chromatographic analysis.

摘要

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