Grenier Y, Ruijs T C, Robitaille Y, Olivier A, Antel J P
Department of Neurology and Neurosurgery, McGill University, Montreal, Que., Canada.
J Neuroimmunol. 1989 Feb;21(2-3):103-15. doi: 10.1016/0165-5728(89)90166-5.
Using immunohistochemical techniques, we examined major histocompatibility complex (MHC) antigen expression on astrocytes, oligodendrocytes, and macrophages-microglia derived from surgically resected tissue from young adults and maintained in dissociated cell cultures supplemented with either fetal calf or human AB serum. The majority of these cells in culture expressed class I MHC antigens. MHC class II expression was observed on only a restricted proportion of astrocytes either under basal or induction conditions (gamma-interferon, activated lymphocyte supernatants), on the majority of macrophages-microglia under inducing conditions, and not on oligodendrocytes. MHC class II expression on astrocytes in culture did not correlate with the extent of in situ gliosis or with in vitro cell morphology. MHC antigen expression was not detected in situ immunohistochemically. These data extend observations on the dissociation of in vivo and in vitro expression of MHC antigens on glial cells. The apparent greater expression of MHC class II antigens on macrophages-microglia compared to astrocytes raises the issue of the relative roles of each of these cell types in promoting immune reactivity under pathologic conditions.
利用免疫组织化学技术，我们检测了来自年轻成年人手术切除组织并在补充有胎牛血清或人AB血清的解离细胞培养物中培养的星形胶质细胞、少突胶质细胞以及巨噬细胞-小胶质细胞上主要组织相容性复合体（MHC）抗原的表达情况。培养中的这些细胞大多数表达I类MHC抗原。在基础条件或诱导条件下（γ-干扰素、活化淋巴细胞上清液），仅在有限比例的星形胶质细胞上观察到II类MHC表达；在诱导条件下，大多数巨噬细胞-小胶质细胞上有II类MHC表达，而少突胶质细胞上未检测到。培养中的星形胶质细胞上II类MHC表达与原位胶质增生程度或体外细胞形态均无相关性。原位免疫组织化学未检测到MHC抗原表达。这些数据扩展了关于胶质细胞上MHC抗原体内和体外表达解离的观察结果。与星形胶质细胞相比，巨噬细胞-小胶质细胞上II类MHC抗原的明显更高表达引发了在病理条件下每种细胞类型在促进免疫反应中相对作用的问题。
