Simpson W J, Tagg J R
Can J Microbiol. 1983 Oct;29(10):1445-51. doi: 10.1139/m83-221.
All 40 tested isolates of M-type 57 group A streptococci gave the same, highly characteristic inhibitory pattern (P-type 614) when tested by deferred antagonism using a set of nine indicator strains. One component of this inhibitory activity was attributed to the production of a bacteriocinlike substance, streptococcin A-M57 (SA-M57). Production of SA-M57 was enhanced by the presence of blood and also by growth at an alkaline pH. Partially purified SA-M57 was obtained from culture supernatants by a combination of ammonium sulphate fractionation and column chromatography. On Sephadex G-100 two different molecular weight forms (SA-M57 alpha, greater than 100 000; SA-M57 beta, 33 000) were demonstrated. Both SA-M57 forms were protease and heat sensitive and had identical inhibitory activity against a collection of indicator bacteria. The adsorption to and rate of kill of sensitive cells by SA-M57 was enhanced in the presence of human plasma. Partially purified SA-M57 preparations were devoid of M-type 57 protein and curing studies showed that loss of SA-M57 did not correlate with loss of M protein production.
当使用一组9种指示菌株通过延迟拮抗试验进行检测时，所有40株经测试的M57型A群链球菌分离株均呈现相同的、高度特征性的抑制模式（P型614）。这种抑制活性的一个成分归因于一种类细菌素物质——链球菌素A-M57（SA-M57）的产生。血液的存在以及在碱性pH条件下生长可增强SA-M57的产生。通过硫酸铵分级分离和柱色谱相结合的方法从培养上清液中获得了部分纯化的SA-M57。在葡聚糖G-100上显示出两种不同分子量形式（SA-M57α，大于100 000；SA-M57β，33 000）。两种SA-M57形式均对蛋白酶和热敏感，并且对一组指示细菌具有相同的抑制活性。在人血浆存在的情况下，SA-M57对敏感细胞的吸附和杀灭速率增强。部分纯化的SA-M57制剂不含M57型蛋白，治愈研究表明SA-M57的丧失与M蛋白产生的丧失无关。
