Murray H G
J Hyg (Lond). 1984 Feb;92(1):117-24. doi: 10.1017/s0022172400064093.
A micro test for the detection of rubella IgG antibody by passive haemolysis in free solution (MIH) is presented. The technique is reproducible, sensitive, simple to perform and economical in the use of reagents. Identical results were obtained in all but one of 408 tests on serum specimens screened for rubella antibody at 15 i.u./ml by the MIH and haemagglutination inhibition (HI) techniques. The quantitative potential of the MIH test and the relative merits of the MIH, HI and radial haemolysis (RH) procedures for rubella screening are discussed.
本文介绍了一种用于在游离溶液中通过被动溶血检测风疹IgG抗体的微量试验(MIH)。该技术具有可重复性、敏感性,操作简单且试剂使用经济。通过MIH和血凝抑制(HI)技术对408份血清标本进行风疹抗体筛查,浓度为15国际单位/毫升,除一份标本外,其余所有标本的检测结果均相同。文中还讨论了MIH试验的定量潜力以及MIH、HI和放射状溶血(RH)方法在风疹筛查中的相对优点。