针对细胞表面抗原的单克隆抗体的检测：与红细胞偶联的抗球蛋白的应用。
Detection of monoclonal antibodies against cell surface antigens: the use of antiglobulins coupled to red blood cells.
作者信息
Clark M R, Cobbold S P, Waldmann H, Coombs R R
出版信息
J Immunol Methods. 1984 Jan 20;66(1):81-7. doi: 10.1016/0022-1759(84)90250-3.
An antiglobulin-coupled red cell assay is described for screening monoclonal antibodies against cell surface antigens. A monoclonal antibody specific for rat immunoglobulin kappa chains was coupled to red blood cells and used to detect binding of rat monoclonal antibodies to cells attached to the wells of microtitre plates. The method was found to be simpler and more rapid than the alternative enzyme-linked binding assay and useful for rapid screening and selection of antibodies for use as differentiation markers of human and mouse haemopoietic cells.
描述了一种抗球蛋白偶联红细胞测定法，用于筛选针对细胞表面抗原的单克隆抗体。一种对大鼠免疫球蛋白κ链具有特异性的单克隆抗体与红细胞偶联，并用于检测大鼠单克隆抗体与附着在微量滴定板孔中的细胞的结合。发现该方法比替代的酶联结合测定法更简单、更快速，并且可用于快速筛选和选择用作人和小鼠造血细胞分化标志物的抗体。
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