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Identification of immunoglobulin class and subclass of mouse monoclonal antibodies to human cell surface antigens by mixed hemadsorption assay.

作者信息

Liao S K, Kwong P C, Dent P B, Clarke B J

出版信息

J Immunoassay. 1981;2(3-4):227-38. doi: 10.1080/15321818108056979.

DOI:10.1080/15321818108056979
PMID:7047572
Abstract

A microassay is described to determine immunoglobulin Ig class or IgG subclass of mouse monoclonal antibodies by mixed hemadsorption assay. Monoclonal antibody bound to adherent target cells is reacted with serial dilutions of a panel of class or subclass specific rabbit anti-mouse Ig antisera and binding of the latter is traced by anti-rabbit globulin-coated indicator erythrocytes. The class or subclass of the bound monoclonal antibody is revealed by preferential binding of the corresponding rabbit antibody. Unlike gel immunodiffusion analysis, the mixed hemadsorption assay may be performed with unconcentrated hybridoma culture supernatants.

摘要

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