Louis J C, Langley K, Anglard P, Wolf M, Vincendon G
Neurosci Lett. 1983 Nov 11;41(3):313-9. doi: 10.1016/0304-3940(83)90469-x.
A method for cultivating neurones from the fetal human central nervous system in the absence of glial cells is described. Brain cells from 15-18-week-old human fetuses are plated on polylysine-coated surfaces and grown in a serum-free hormonally-defined medium. About 98% of the cells were identified as neurones using tetanus toxin as a marker. The cultures survive for up to 7 weeks and develop an extremely complex network of neurites.
本文描述了一种在无胶质细胞的情况下从人类胎儿中枢神经系统培养神经元的方法。将15至18周龄人类胎儿的脑细胞接种在聚赖氨酸包被的表面，并在无血清激素限定培养基中培养。使用破伤风毒素作为标志物，约98%的细胞被鉴定为神经元。培养物可存活长达7周，并形成极其复杂的神经突网络。
