Ishii K, Katayama M, Hori K, Yodoi J, Nakanishi T
Department of Cell Biology, Kyoto University, Japan.
Neurosci Lett. 1993 Dec 12;163(2):159-62. doi: 10.1016/0304-3940(93)90371-q.
Effects of 2-mercaptoethanol on primary cultures of fetal mouse brain neurons have been investigated. The addition of 2-mercaptoethanol to the culture medium increased 6- or 200-fold the survival rates of embryonic day-16 murine striatum neurons and day-18 cerebral cortical neurons cultured in serum-free medium, respectively, and also induced neurite outgrowth, particularly being prominent in cortical neurons. Moreover, this drug enhanced trophic activities of the conditioned medium of VR-2g or BIM cells. These findings indicate that 2-mercaptoethanol can support the viability and differentiation of fetal mouse brain neurons.
已对2-巯基乙醇对胎鼠脑神经元原代培养物的影响进行了研究。向培养基中添加2-巯基乙醇分别使在无血清培养基中培养的胚胎第16天小鼠纹状体神经元和第18天大脑皮质神经元的存活率提高了6倍或200倍，并且还诱导了神经突生长，在皮质神经元中尤为突出。此外，这种药物增强了VR-2g或BIM细胞条件培养基的营养活性。这些发现表明，2-巯基乙醇可以支持胎鼠脑神经元的活力和分化。
