[Effect of temperature on exoprotease and bacitracin synthesis and sporulation in Bacillus licheniformis 28KA].

By means of physiological experiments the effect of temperatures ranging from 30 degrees C to 55 degrees C on the synthesis of exoprotease and bacitracin, as well as on sporification in Bacillus licheniformis 28KA was studied. The synthesis of exoprotease and sporification were found to be less stable to the temperature variation than the vegetative growth of the microorganism. The temperature, favourable for the maximum yield of then enzyme and spores, is 10 degrees below the temperature which is optimal for the growth of the organism (50 degrees C). The synthesis of bacitracin is substantially sensitive to the temperature variation. The maximum synthesis of the antibiotic was observed at 50 degrees C. High concentrations of glucose inhibit the synthesis of the enzyme and bacitracin, as well as sporification in Bacillus licheniformis 28KA.