Lee S C, Beery J T, Chu F S
Toxicol Appl Pharmacol. 1984 Feb;72(2):228-35. doi: 10.1016/0041-008x(84)90307-7.
Antibody against T-2 toxin was used for monitoring the fate of T-2 toxin in mice given a single po dose of 11 mg/kg by the peroxidase-antiperoxidase (PAP) method. T-2 toxin was demonstrable in the esophagus from 5 min to about 24 hr postdosing. In the stomach, T-2 toxin was detected within the cytoplasm of intact and injured epithelial cells. In the duodenum, T-2 toxin was primarily localized within the surface epithelium and phagocytic elements (macrophages and neutrophils) of the duodenal lamina propria, especially toward the tips of the villi. Following sloughing of duodenal villous tips, the recovering villous tip epithelial cells frequently showed both cytoplasmic and nuclear T-2 toxin. The jejunum showed weak T-2 toxin within the cytoplasm of villous tip epithelial cells only. The ileum never demonstrated T-2 toxin. Tissue response in the gastrointestinal (GI) tract was characterized by transient edema, marked cytolysis and sloughing, and a subsequent leukocytic invasion of the stomach and proximal small intestine. Evidence of severe gastric and less severe duodenal bleeding was apparent and associated with a marked loss of gastric epithelium and intestinal villous tips. The kidney medulla contained the majority of T-2 toxin stain. T-2 toxin was noted within the distal tubular cells, the cells of the collecting tubules, and the epithelium covering the papilla. T-2 toxin was never demonstrated in any of the hepatic tissue examined.
采用过氧化物酶抗过氧化物酶（PAP）法，使用抗T-2毒素抗体监测单次经口给予11 mg/kg剂量T-2毒素的小鼠体内T-2毒素的去向。给药后5分钟至约24小时，在食管中可检测到T-2毒素。在胃中，完整和受损上皮细胞的细胞质内均可检测到T-2毒素。在十二指肠中，T-2毒素主要定位于十二指肠固有层的表面上皮和吞噬细胞（巨噬细胞和中性粒细胞），尤其是在绒毛尖端。十二指肠绒毛尖端脱落后，恢复中的绒毛尖端上皮细胞的细胞质和细胞核中常显示有T-2毒素。空肠仅在绒毛尖端上皮细胞的细胞质内显示弱阳性的T-2毒素。回肠从未检测到T-2毒素。胃肠道（GI）的组织反应表现为短暂性水肿、明显的细胞溶解和脱落，随后白细胞侵入胃和近端小肠。明显可见严重的胃出血和不太严重的十二指肠出血，且与胃上皮和肠绒毛尖端的明显丧失有关。肾髓质含有大部分T-2毒素染色。在远端肾小管细胞、集合小管细胞以及覆盖乳头的上皮中可发现T-2毒素。在所检查的任何肝组织中均未检测到T-2毒素。
