Identification of the purC gene product of Escherichia coli.
作者信息
Parker J
出版信息
J Bacteriol. 1984 Mar;157(3):712-7. doi: 10.1128/jb.157.3.712-717.1984.
Abstract
The purC region of the Escherichia coli chromosome was isolated from in vivo-derived lambda transducing bacteriophages and cloned in high-copy-number plasmids. The product of the purC gene, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, was identified as a protein with an Mr of ca. 27,000. The level of the protein is increased by more than 60-fold in strains carrying the gene on a high-copy-number plasmid. Purine addition represses the enzyme level in both plasmid- and non-plasmid-containing strains.
摘要
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