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大肠杆菌purC基因产物的鉴定。

Identification of the purC gene product of Escherichia coli.

作者信息

Parker J

出版信息

J Bacteriol. 1984 Mar;157(3):712-7. doi: 10.1128/jb.157.3.712-717.1984.

DOI:10.1128/jb.157.3.712-717.1984
PMID:6365889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215316/
Abstract

The purC region of the Escherichia coli chromosome was isolated from in vivo-derived lambda transducing bacteriophages and cloned in high-copy-number plasmids. The product of the purC gene, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, was identified as a protein with an Mr of ca. 27,000. The level of the protein is increased by more than 60-fold in strains carrying the gene on a high-copy-number plasmid. Purine addition represses the enzyme level in both plasmid- and non-plasmid-containing strains.

摘要

从体内衍生的λ转导噬菌体中分离出大肠杆菌染色体的purC区域，并将其克隆到高拷贝数质粒中。purC基因的产物，磷酸核糖氨基咪唑琥珀酰胺羧酰胺合成酶，被鉴定为一种分子量约为27,000的蛋白质。在携带该基因的高拷贝数质粒的菌株中，该蛋白质的水平增加了60多倍。添加嘌呤会抑制含质粒和不含质粒的菌株中的酶水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0c4/215316/15d4e3fe6f78/jbacter00238-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0c4/215316/db70e49a71b3/jbacter00238-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0c4/215316/15d4e3fe6f78/jbacter00238-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0c4/215316/db70e49a71b3/jbacter00238-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0c4/215316/15d4e3fe6f78/jbacter00238-0027-a.jpg

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本文引用的文献

1
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
2
Regulation of purE transcription in a purE::lac fusion strain of Escherichia coli.大肠杆菌purE::lac融合菌株中purE转录的调控
J Bacteriol. 1982 Mar;149(3):1041-9. doi: 10.1128/jb.149.3.1041-1049.1982.
3
Construction and expression of hybrid plasmids containing the Escherichia coli glyA genes.含有大肠杆菌glyA基因的杂交质粒的构建与表达。
J Bacteriol. 1986 Apr;166(1):297-300. doi: 10.1128/jb.166.1.297-300.1986.
4
Linkage map of Escherichia coli K-12, edition 8.大肠杆菌K-12连锁图谱，第8版。
Microbiol Rev. 1990 Jun;54(2):130-97. doi: 10.1128/mr.54.2.130-197.1990.
5
DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12.编码5'-磷酸核糖基-5-氨基咪唑-4-N-琥珀酰胺羧酰胺合成酶的purC基因的DNA序列及大肠杆菌K-12的dapA-purC区域的组织架构
J Bacteriol. 1990 Oct;172(10):6035-41. doi: 10.1128/jb.172.10.6035-6041.1990.
6
A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome.大肠杆菌染色体dapA-purC区间内一种新脂蛋白的基因。
J Bacteriol. 1991 Sep;173(17):5523-31. doi: 10.1128/jb.173.17.5523-5531.1991.
7
Cloning, characterization, and expression of the dapE gene of Escherichia coli.大肠杆菌dapE基因的克隆、特性分析及表达
J Bacteriol. 1992 Aug;174(16):5265-71. doi: 10.1128/jb.174.16.5265-5271.1992.
Gene. 1981 Jun-Jul;14(1-2):63-72. doi: 10.1016/0378-1119(81)90148-7.
4
A DNA-binding protein with specificity for pur genes in Escherichia coli.一种对大肠杆菌中嘌呤基因具有特异性的DNA结合蛋白。
J Biol Chem. 1980 Oct 25;255(20):9594-8.
5
Strains of Escherichia coli carrying the structural gene for histidyl-tRNA synthetase on a high copy-number plasmid.携带组氨酰 - tRNA合成酶结构基因的大肠杆菌菌株，该基因位于高拷贝数质粒上。
Mol Gen Genet. 1981;183(1):115-22. doi: 10.1007/BF00270148.
6
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J Bacteriol. 1983 Feb;153(2):837-45. doi: 10.1128/jb.153.2.837-845.1983.
7
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Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
8
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9
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J Biol Chem. 1982 Apr 10;257(7):3525-31.
10
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.