Barrett E L, Collins E B, Hall B J, Matoi S H
J Dairy Sci. 1983 Dec;66(12):2507-14. doi: 10.3168/jds.S0022-0302(83)82119-5.
Production of 2,3-butylene glycol from whey with Klebsiella pneumoniae and Enterobacter aerogenes was studied. Sterilization of the whey was unnecessary. Acid whey required neutralization, but sweet whey did not. Butylene glycol production was most efficient at 33 degrees C for Klebsiella pneumoniae and at 37 degrees C for Enterobacter aerogenes. Aeration significantly improved yields. Klebsiella pneumoniae produced more butylene glycol than did Enterobacter aerogenes in unsupplemented whey. The addition of 50 mM sodium acetate to whey increased the production of butylene glycol and acetoin by Enterobacter aerogenes; it also increased the production of glycol by Klebsiella pneumoniae, but the increase in this case was offset by a decrease of production of acetoin. Maximal yields of the glycol plus acetoin in whey were obtained in 48 to 64 h, but Enterobacter aerogenes required about 160 h for complete utilization of the lactose. Highest yields were about .3 M butylene glycol plus acetoin, which corresponds to the production of about 10 kg of glycol from 380 liters of whey.
研究了肺炎克雷伯菌和产气肠杆菌利用乳清生产2,3-丁二醇的情况。乳清无需灭菌。酸乳清需要中和，但甜乳清则不需要。肺炎克雷伯菌在33℃时丁二醇产量最高，产气肠杆菌在37℃时产量最高。通气显著提高了产量。在未添加其他成分的乳清中，肺炎克雷伯菌产生的丁二醇比产气肠杆菌多。向乳清中添加50 mM醋酸钠可增加产气肠杆菌丁二醇和3-羟基丁酮的产量；它也增加了肺炎克雷伯菌二醇的产量，但在这种情况下，产量的增加被3-羟基丁酮产量的下降所抵消。在48至64小时内可获得乳清中二醇和3-羟基丁酮的最大产量，但产气肠杆菌完全利用乳糖需要约160小时。最高产量约为0.3 M丁二醇加3-羟基丁酮，这相当于从380升乳清中生产约10千克二醇。
