Bröcker E B, Suter L, Sorg C
J Invest Dermatol. 1984 Mar;82(3):244-7. doi: 10.1111/1523-1747.ep12260181.
Ninety-three primary malignant melanomas of the skin were typed immunohistologically for the expression of HLA-DR on tumor cells. HLA-DR-positive stroma cells were HLA-DR-negative or only locally positive in most cases. In 36 (39%) of the lesions more than 10% of the tumor cells were stained by two monoclonal antibodies against the nonpolymorphic portion of HLA-DR. HLA-DR-positive tumor cells were often accumulated at the advancing front of the melanoma. The occurrence of HLA-DR-positive tumor cells was related to tumor thickness and level of invasion. Substantial numbers of HLA-DR-positive tumor cells were found in half of the tumors thicker than 1.5 mm and in only 18% of flatter lesions. The highest percentage of HLA-DR-positive tumors was found in the group of melanomas invading the reticular dermis (level IV). The majority of tumors (18/24) that had metastasized within an observation period of 0-32 months were HLA-DR-positive. Regarding the mononuclear cell infiltrate, no correlation between the degree of overall infiltrate and the expression of HLA-DR by the tumor cells was found. The infiltrate within the tumor, however, was more often marked in HLA-DR-positive than in HLA-DR-negative melanomas.
对93例原发性皮肤恶性黑色素瘤进行免疫组织化学分型，以检测肿瘤细胞上HLA - DR的表达。在大多数情况下，HLA - DR阳性的基质细胞HLA - DR阴性或仅局部阳性。在36例（39%）病变中，超过10%的肿瘤细胞被两种针对HLA - DR非多态性部分的单克隆抗体染色。HLA - DR阳性的肿瘤细胞常聚集在黑色素瘤的进展前沿。HLA - DR阳性肿瘤细胞的出现与肿瘤厚度和浸润程度有关。在厚度超过1.5 mm的肿瘤中，半数发现大量HLA - DR阳性肿瘤细胞，而在较扁平病变中仅18%有此情况。HLA - DR阳性肿瘤的最高比例见于侵犯网状真皮的黑色素瘤组（IV级）。在0 - 32个月观察期内发生转移的大多数肿瘤（18/24）为HLA - DR阳性。关于单核细胞浸润，未发现总体浸润程度与肿瘤细胞HLA - DR表达之间存在相关性。然而，肿瘤内的浸润在HLA - DR阳性黑色素瘤中比在HLA - DR阴性黑色素瘤中更常见。
