Sasagawa M, Suzuki T, Kajino T, Ohno M, Kanazawa K, Takeuchi S
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Jan;36(1):81-4.
Choriocarcinoma can be regarded as transplanted cancer, because its origin is in trophoblast which is fetal tissue. It is of interest whether HLA antigens are expressed on choriocarcinoma cells or not, since HLA antigens may play an important role if the patient's body could recognize choriocarcinoma as "not self" and initiate its immune response. In this report choriocarcinoma tissue in the uterus, obtained after hysterectomy, is stained by an indirect immunofluorescence technique using monoclonal antibodies to HLA-A,B,C and HLA-DR. We believe this is the first immunohistological study in which choriocarcinoma in utero is available for study and monoclonal antibodies to HLA antigens are applied. In the staining of HLA-A,B,C, and of HLA-DR, host cells, such as myometrial cells, show positive staining but choriocarcinoma cells show negative staining. It is thought that choriocarcinoma cells are viable and show negative staining of HLA antigens, on the basis of finding following HE staining and hCG staining using anti-hCG antibody and the clinical remarks of the patient such as her high urinary hCG titer before operation and no history of chemotherapy. These results agree with those of previous studies about HLA antigens on choriocarcinoma cell lines.
绒毛膜癌可被视为移植性癌，因为其起源于作为胎儿组织的滋养层。如果患者的身体能够将绒毛膜癌识别为“非自身”并启动免疫反应，那么人类白细胞抗原（HLA）抗原是否在绒毛膜癌细胞上表达就成为一个有趣的问题。在本报告中，采用针对HLA - A、B、C和HLA - DR的单克隆抗体，通过间接免疫荧光技术对子宫切除术后获得的子宫内绒毛膜癌组织进行染色。我们认为这是首次对子宫内绒毛膜癌进行研究并应用针对HLA抗原的单克隆抗体的免疫组织学研究。在HLA - A、B、C以及HLA - DR的染色中，宿主细胞如子宫肌层细胞呈现阳性染色，而绒毛膜癌细胞呈现阴性染色。基于苏木精 - 伊红（HE）染色、使用抗人绒毛膜促性腺激素（hCG）抗体的hCG染色结果以及患者术前高尿hCG滴度且无化疗史等临床情况，认为绒毛膜癌细胞是存活的且HLA抗原呈阴性染色。这些结果与先前关于绒毛膜癌细胞系上HLA抗原的研究结果一致。
