Travers P J, Arklie J L, Trowsdale J, Patillo R A, Bodmer W F
Natl Cancer Inst Monogr. 1982;60:175-80.
We studied a panel of 21 human tumor and other cell lines, mainly of epithelial origin, for the expression of HLA-ABC antigens by an indirect radiobinding assay using monoclonal antibodies. Four of the lines, 2 choriocarcinomas, a breast tumor cell lin (MDA-MB-157), and a colon carcinoma (LoVo) showed no detectable reaction with the antibodies. The levels of binding in another breast tumor line (SkBr3) and a colon carcinoma line (LS174T) were much reduced. These cell lines plus some of the HLA-ABC positive lines from the panel were studied by immunoprecipitation of 35S-labeled cell extracts. Two mechanisms for alteration of the HLA-ABC antigen levels at the cell surface were apparent. In the choriocarcinomas and one breast tumor line, beta 2 microglobulin (beta 2m) was expressed but little or no HLA-A, B, or C polypeptides were synthesized. The other HLA-ABC negative lines had defects similar to that in the previously reported Burkitt's lymphoma cell line DAUDI. In these lines, HLA-ABC polypeptides were produced but did not appear at the cell surface because beta 2m was not synthesized. The absence of HLA-ABC determinants from the cell surface is presumed to afford protection against T-cell immune attack whether naturally as against the trophoblast during pregnancy or in abnormal situations against certain tumors.
我们使用单克隆抗体通过间接放射结合试验研究了一组21种人类肿瘤及其他细胞系（主要为上皮来源）中HLA - ABC抗原的表达情况。其中有4种细胞系，2种绒毛膜癌、1种乳腺肿瘤细胞系（MDA - MB - 157）和1种结肠癌细胞系（LoVo）与抗体未显示出可检测到的反应。另一种乳腺肿瘤细胞系（SkBr3）和一种结肠癌细胞系（LS174T）的结合水平则大幅降低。通过对35S标记的细胞提取物进行免疫沉淀，研究了这些细胞系以及该组中一些HLA - ABC阳性细胞系。细胞表面HLA - ABC抗原水平改变的两种机制显而易见。在绒毛膜癌和一种乳腺肿瘤细胞系中，β2微球蛋白（β2m）表达，但几乎没有合成HLA - A、B或C多肽。其他HLA - ABC阴性细胞系具有与先前报道的伯基特淋巴瘤细胞系DAUDI类似的缺陷。在这些细胞系中，HLA - ABC多肽产生但未出现在细胞表面，因为未合成β2m。细胞表面缺乏HLA - ABC决定簇被认为可提供针对T细胞免疫攻击的保护，无论是在自然情况下如孕期针对滋养层，还是在异常情况下针对某些肿瘤。
