Juretić D, Lipovac K, Hadzija M, Slijepcević M
J Clin Chem Clin Biochem. 1984 Jan;22(1):21-7. doi: 10.1515/cclm.1984.22.1.21.
The catalytic activities of N-acetyl-beta-D-glucosaminidase, beta-galactosidase and alpha-glucosidase in kidney and urine of diabetic rats were investigated in relation to the duration of diabetes, to the degree of constant hyperglycaemia and to the therapeutic control in the early stage of disease. The results suggest that the degree of constant hyperglycaemia and the duration of untreated diabetes are significant determining factors for the course of morphological changes. These changes are manifested as a decrease of the glycosidases in kidney (0.5 to 0.6 time the age-matched controls) and as moderate to severe enzymurias. Daily variation of blood glucose with inadequate insulin Lente therapy caused decreased N-acetyl-beta-D-glucosaminidase and beta-galactosidase activities in kidney as well as enzymuria. Since such changes can be correlated with histologically visible changes in the kidney, the measurement of these enzymes in urine is a simple way of monitoring the development of kidney damage in poorly controlled diabetes. When constant normoglycaemia was maintained for three weeks with insulin Ultralente in diabetic rats with a confirmed decrease of kidney glycosidases, the persisting morphological alteration of the organ was reflected by a urinary output of N-acetyl-beta-D-glucosaminidase.
研究了糖尿病大鼠肾脏和尿液中N - 乙酰 - β - D - 氨基葡萄糖苷酶、β - 半乳糖苷酶和α - 葡萄糖苷酶的催化活性与糖尿病病程、持续性高血糖程度以及疾病早期治疗控制情况的关系。结果表明，持续性高血糖程度和未治疗糖尿病的病程是形态学变化进程的重要决定因素。这些变化表现为肾脏中糖苷酶减少（为年龄匹配对照组的0.5至0.6倍）以及中度至重度酶尿。胰岛素Lente治疗不足导致血糖每日波动，进而引起肾脏中N - 乙酰 - β - D - 氨基葡萄糖苷酶和β - 半乳糖苷酶活性降低以及酶尿。由于这些变化与肾脏组织学可见变化相关，因此检测尿液中的这些酶是监测控制不佳的糖尿病患者肾脏损伤发展的一种简单方法。当用胰岛素Ultralente使糖尿病大鼠持续维持正常血糖三周，且已证实肾脏糖苷酶减少时，器官持续的形态学改变通过N - 乙酰 - β - D - 氨基葡萄糖苷酶的尿排出量得以体现。
