链脲佐菌素诱导的糖尿病中国仓鼠（黑线仓鼠）糖水解酶活性的变化

Alterations in glycohydrolase activities in streptozotocin-diabetic Chinese hamsters (Cricetulus griseus).

Chang A Y, Perry C S, Wyse B M

Diabetes and Atherosclerosis Research, Upjohn Company, Kalamazoo, Michigan 49001.

Comp Biochem Physiol B. 1979;63(3):341-4. doi: 10.1016/0305-0491(79)90259-1.

Abstract

Six weeks after the injection of streptozotocin at 125 mg/kg i.p. in the AV line nondiabetic Chinese hamsters, the animals showed hyperglycemia, increased kidney, pancreas and stomach weights and stomach glucagon contents and depletion of insulin and glucagon in the pancreas. 2. Plasma beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase were elevated; whereas alpha-D-glucosidase was decreased and alpha-D-galactosidase remained unchanged in the plasma. 3. In the kidney, streptozotocin-diabetes led to depression of alpha-D-mannosidase, beta-D-fucosidase and N-acetyl-beta-D-glucosaminidase activities in both 12,000 g supernatant and precipitate fractions, decreases in alpha-D-glucosidase in the supernatant only and no change in alpha-L-fucosidase, alpha-D-galactosidase, beta-D-galactosidase and beta-D-glucuronidase. 4. In the liver, significant increases in N-acetyl-beta-D-glucosaminidase, alpha-D-galactosidase, beta-D-galactosidase, beta-D-fucosidase, beta-D-glucosidase and alpha-D-mannosidase were found in either the supernatant or the precipitate fraction of the diabetic animals. The data indicate diabetes-dependent tissue-specific changes in glycohydrolases in the Chinese hamster.

摘要

给AV系非糖尿病中国仓鼠腹腔注射125mg/kg链脲佐菌素六周后，动物出现高血糖、肾脏、胰腺和胃重量增加、胃胰高血糖素含量增加以及胰腺中胰岛素和胰高血糖素耗竭。2. 血浆β-D-半乳糖苷酶和N-乙酰-β-D-氨基葡萄糖苷酶升高；而血浆中α-D-葡萄糖苷酶降低，α-D-半乳糖苷酶保持不变。3. 在肾脏中，链脲佐菌素诱导的糖尿病导致12,000g上清液和沉淀部分中的α-D-甘露糖苷酶、β-D-岩藻糖苷酶和N-乙酰-β-D-氨基葡萄糖苷酶活性降低，仅上清液中的α-D-葡萄糖苷酶降低，而α-L-岩藻糖苷酶、α-D-半乳糖苷酶、β-D-半乳糖苷酶和β-D-葡萄糖醛酸苷酶无变化。4. 在肝脏中，糖尿病动物的上清液或沉淀部分中，N-乙酰-β-D-氨基葡萄糖苷酶、α-D-半乳糖苷酶、β-D-半乳糖苷酶、β-D-岩藻糖苷酶、β-D-葡萄糖苷酶和α-D-甘露糖苷酶均显著增加。数据表明中国仓鼠中糖水解酶存在糖尿病依赖性组织特异性变化。