Dales R B, Moorhouse J, Croft J H
J Gen Microbiol. 1983 Dec;129(12):3637-42. doi: 10.1099/00221287-129-12-3637.
The heterokaryon incompatibility system in Aspergillus nidulans has been investigated by parasexual methods. The use of complementary auxotrophs with a repeated serial transfer method or with a protoplast fusion technique has enabled heterokaryons and diploid strains to be recovered from heterokaryon incompatible combinations of strains. The effects of allelic interaction at heterokaryon incompatibility (het) loci on the morphologies of the heterokaryon and diploid colonies isolated are described. Parasexual analyses conducted among strains belonging to the heterokaryon compatibility groups, h-cGl and h-cB, and the two recombinant compatibility classes, have located the hetA and hetB genes to linkage groups V and VI respectively.
已通过准性生殖方法研究了构巢曲霉中的异核体不相容系统。使用互补营养缺陷型菌株，采用重复连续转移法或原生质体融合技术，已能够从菌株的异核体不相容组合中获得异核体和二倍体菌株。描述了异核体不相容（het）位点上等位基因相互作用对分离出的异核体和二倍体菌落形态的影响。在属于异核体相容组h-cGl和h-cB以及两个重组相容类别的菌株之间进行的准性分析，已分别将hetA和hetB基因定位到连锁群V和VI。
