Dales R B, Croft J H
J Gen Microbiol. 1983 Dec;129(12):3643-9. doi: 10.1099/00221287-129-12-3643.
Protoplast fusion has made possible the isolation of a diploid strain from haploid parents belonging to heterokaryon compatibility (h-c) groups Q and Gl of Aspergillus nidulans. This diploid was not fully heterokaryon compatibility tests conducted between selected pairs of parasexually derived progeny strains facilitated a chromosome assay method for the detection of heterokaryon incompatibility (het) genes. Despite the lack of segregation for the linkage group VI marker, it proved possible to locate het genes on linkage groups III, V, VI and VII. Backcross data detected five het gene differences operating between the h-cQ and h-cGl parental strains. Two het loci were located on linkage group III.
原生质体融合使得从属于构巢曲霉异核体相容性（h-c）Q组和Gl组的单倍体亲本中分离出二倍体菌株成为可能。这个二倍体并不完全是异核体相容性的。在选定的部分准性生殖衍生后代菌株对之间进行的相容性测试促进了一种用于检测异核体不相容性（het）基因的染色体分析方法。尽管连锁群VI标记没有发生分离，但事实证明可以将het基因定位在连锁群III、V、VI和VII上。回交数据检测到在h-cQ和h-cGl亲本菌株之间起作用的5个het基因差异。两个het位点位于连锁群III上。
