Ffrench M, Fiere D, Bryon P A, Cordier G, Viala J J
Pathol Biol (Paris). 1984 Feb;32(2):139-43.
DNA and proteins of ethanol fixed cells are simultaneously analysed by cytofluorimetry in 35 cases of adult acute leukemias. DNA is coloured by propidium iodide and proteins by FITC. These technics allow to know the GO/G1, S, G2 + M cell-cycle phases and the protein cell content for each phase. We also can evaluate the fraction of low protein content cells (LPC fraction) that seems to be the GO and early G1 fraction. A good correlation exists between the percentage of S phase cells and the LPC fraction. This parameter, because of its important dispersion, must have a better discriminating value than S phase, especially for the prognosis analysis. Furthermore acute myeloid leukemias have a protein content that varies with the cytological maturation. Therefore, P1 + FITC colorations would give quantitative criteria for the acute leukemias cytological analysis and their prognostic valuation.
采用细胞荧光测定法对35例成人急性白血病乙醇固定细胞的DNA和蛋白质进行了同步分析。用碘化丙啶对DNA进行染色，用异硫氰酸荧光素对蛋白质进行染色。这些技术能够了解G0/G1、S、G2+M细胞周期阶段以及各阶段的蛋白质细胞含量。我们还可以评估低蛋白质含量细胞的比例（LPC比例），其似乎是G0和早期G1比例。S期细胞百分比与LPC比例之间存在良好的相关性。由于该参数具有较大的离散度，其鉴别价值必定优于S期，尤其在预后分析方面。此外，急性髓系白血病的蛋白质含量会随着细胞成熟而变化。因此，P1+异硫氰酸荧光素染色可为急性白血病的细胞学分析及其预后评估提供定量标准。
