Oga A, Murakami T, Kimura Y, Ida M, Takahashi M
Dept. of Pathology, Yamaguchi University School of Medicine.
Gan To Kagaku Ryoho. 1993 Apr;20(6):756-8.
We were successful in obtaining simultaneous measurements of kinetochore protein and DNA with flow cytometry using HeLa cells and KM cells; the latter originated with a squamous cell carcinoma of the oral cavity. Kinetochore protein was stained with the serum obtained from a patient with CREST syndrome and FITC-conjugated goat antihuman IgG antibody; the DNA was stained with propidium iodide. The FITC fluorescence of cells with a large DNA index was stronger than in cells with a small DNA index. The FITC intensity increased from G1 phase to G2/M phase. The rate of FITC intensity increase representing the kinetochore protein amount was greater in the early and late S phases than in the other phases. The results suggest that kinetochore protein increases in the interphase as the cell cycle unfolds.
我们成功地使用HeLa细胞和KM细胞（后者源自口腔鳞状细胞癌），通过流式细胞术同时测量了动粒蛋白和DNA。动粒蛋白用从CREST综合征患者获得的血清和异硫氰酸荧光素（FITC）偶联的山羊抗人IgG抗体进行染色；DNA用碘化丙啶染色。DNA指数大的细胞的FITC荧光比DNA指数小的细胞更强。FITC强度从G1期到G2/M期增加。代表动粒蛋白量的FITC强度增加率在S期早期和晚期比其他时期更大。结果表明，随着细胞周期的展开，动粒蛋白在间期增加。
