合成生长调节素C：与从人血浆中分离出的天然激素的比较。
Synthetic somatomedin C: comparison with natural hormone isolated from human plasma.
作者信息
Van Wyk J J, Russell W E, Li C H
出版信息
Proc Natl Acad Sci U S A. 1984 Feb;81(3):740-2. doi: 10.1073/pnas.81.3.740.
Abstract
The biological and immunological properties of a chemically synthesized preparation of somatomedin C (Sm-C) were compared with those of the natural product isolated from human plasma. The two preparations produced identical curves in the radioimmunoassay and radioreceptor assay for Sm-C and in the radioreceptor assay for insulin. They were identical in their ability to stimulate DNA synthesis in confluent BALB/c 3T3 cells previously exposed to platelet-derived growth factor, and the biological activities of both preparations were completely neutralized by a monoclonal antibody raised against native Sm-C. These studies demonstrate that the chemically synthesized product is equivalent to the native molecule in all important respects and that it can be used interchangeably with the natural product for any studies that are contemplated.
摘要
将化学合成的生长调节素C（Sm-C）制剂的生物学和免疫学特性与从人血浆中分离出的天然产物的特性进行了比较。这两种制剂在Sm-C的放射免疫测定和放射受体测定以及胰岛素的放射受体测定中产生了相同的曲线。它们在刺激先前暴露于血小板衍生生长因子的汇合BALB/c 3T3细胞中DNA合成的能力方面是相同的，并且两种制剂的生物活性都被针对天然Sm-C产生的单克隆抗体完全中和。这些研究表明，化学合成产物在所有重要方面都等同于天然分子，并且在任何预期的研究中都可以与天然产物互换使用。
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