胰岛素样生长因子I（生长调节素C）的全合成。
Total synthesis of insulin-like growth factor I (somatomedin C).
作者信息
Li C H, Yamashiro D, Gospodarowicz D, Kaplan S L, Van Vliet G
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2216-20. doi: 10.1073/pnas.80.8.2216.
Abstract
Human insulin-like growth factor I (somatomedin C) with 70 amino acid residues and three disulfide bridges has been synthesized by the solid-phase method. The synthetic product behaves as a pure polypeptide in paper electrophoresis, isoelectric focusing, and high-performance liquid chromatography, Its elution behavior in Sephadex G-50, isoelectric point, amino acid composition, and growth-stimulating activity in bovine adrenal cortical cells or granulosa cells are comparable to those reported for the natural product. In radioimmunoassay, the synthetic product is indistinguishable from the natural hormone when either synthetic or natural product is used as the labeled ligand.
摘要
具有70个氨基酸残基和三个二硫键的人胰岛素样生长因子I（生长调节素C）已通过固相法合成。合成产物在纸电泳、等电聚焦和高效液相色谱中表现为纯多肽。其在葡聚糖凝胶G - 50中的洗脱行为、等电点、氨基酸组成以及在牛肾上腺皮质细胞或颗粒细胞中的生长刺激活性与天然产物报道的相当。在放射免疫测定中，当使用合成产物或天然产物作为标记配体时，合成产物与天然激素无法区分。
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