[Isolation and quantitative analysis of ATP, synthesized by preparations of enriched plasma membrane portions from various tissues in the presence of insulin and somatotropin].

A transiently existent ATP, synthesized by the preparation enriched with plasmatic membranes of particles from rat skeletal muscles in presence of insulin 4 micrograms/ml and somatotropin 10-22 micrograms/ml within 1 min after addition of the hormones at 30 degrees, was isolated by means of chromatography on Dowex 1X8. The ATP was synthesized in a medium containing Tris-HCl buffer, pH 7.5, ADP, Mg2+ and P1 during NADH-dependent oxidation in presence of cytochrome c and oxygen; amount of the ATP was 0.1-0.3 nmole/mg/min. This form of ATP was isolated from particles enriched with plasmatic membranes and from plasmatic membranes of rat adipocytes, lymphocytes and liver tissue as well as of human erythrocytes within 1 min after addition of insulin into the mixture. The highest insulin-stimulated ATP-producing activity was found in plasmatic membranes of rat adipocytes--7.45 nmole/mg/min. Radiolabelled ATP, in presence of insulin transiently synthesized from ADP and 32P, was isolated from the preparation of human erythrocyte plasmatic membranes. Conditions of insulin-stimulated formation of the transient ATP in the preparation of human erythrocyte plasmatic membranes were studied.