Foley J E, Kashiwagi A, Chang H, Huecksteadt T P, Lillioja S, Verso M A, Reaven G
Am J Physiol. 1984 Mar;246(3 Pt 1):E211-5. doi: 10.1152/ajpendo.1984.246.3.E211.
In an effort to determine whether differences in basal and maximum insulin-stimulated glucose transport by isolated adipocytes are a function of donor sex, we measured glucose transport rates in the absence and presence of 8 nM insulin in adipocytes isolated from the abdominal subcutaneous fat tissue of nine male and ten female subjects with varying degrees of obesity and in adipocytes isolated from the abdominal subcutaneous and retroperitoneal fat tissue of (180-220 g) male and female rats. Because maximal insulin-stimulated glucose transport rate per cell of adipocytes isolated from subcutaneous abdominal tissue of male and female subjects was constant in each sex, the data have been normalized on the basis of transport per cell. The results demonstrated that basal and maximal insulin-stimulated glucose transport per cell was 53-75% higher per cell in the females versus males in adipocytes from human subcutaneous abdominal adipose tissue (P less than 0.01). A similar difference in glucose transport rate between males and females (P less than 0.001) was also found in rat abdominal subcutaneous adipose tissue. Adipocytes isolated from rat retroperitoneal adipose tissue had higher transport rates (approximately three-fold) and smaller sex differences (35% higher in females) than found in adipocytes from rat and human subcutaneous tissue. These results indicate that basal and maximum insulin-stimulated glucose transport is higher by adipocytes isolated from females and that this difference is independent of adipose cell size and species.
为了确定分离的脂肪细胞基础胰岛素刺激的葡萄糖转运以及最大胰岛素刺激的葡萄糖转运差异是否取决于供体性别，我们测量了从9名男性和10名不同肥胖程度女性的腹部皮下脂肪组织分离出的脂肪细胞在不存在和存在8 nM胰岛素时的葡萄糖转运速率，以及从（180 - 220 g）雄性和雌性大鼠的腹部皮下和腹膜后脂肪组织分离出的脂肪细胞的葡萄糖转运速率。由于从男性和女性受试者腹部皮下组织分离出的脂肪细胞每细胞的最大胰岛素刺激葡萄糖转运速率在各性别中是恒定的，因此数据已根据每细胞转运进行了标准化。结果表明，在人类腹部皮下脂肪组织的脂肪细胞中，女性每细胞的基础胰岛素刺激葡萄糖转运和最大胰岛素刺激葡萄糖转运比男性高53 - 75%（P < 0.01）。在大鼠腹部皮下脂肪组织中也发现了雄性和雌性之间类似的葡萄糖转运速率差异（P < 0.001）。从大鼠腹膜后脂肪组织分离出的脂肪细胞比从大鼠和人类皮下组织分离出的脂肪细胞具有更高的转运速率（约三倍）和更小的性别差异（女性高35%）。这些结果表明，从女性分离出的脂肪细胞基础胰岛素刺激葡萄糖转运和最大胰岛素刺激葡萄糖转运更高，并且这种差异与脂肪细胞大小和物种无关。
