Caprioli A, Donelli G, Falbo V, Possenti R, Roda L G, Roscetti G, Ruggeri F M
Biochem Biophys Res Commun. 1984 Jan 30;118(2):587-93. doi: 10.1016/0006-291x(84)91343-3.
A purification procedure for a protein obtained from an pathogenic strain of E. coli is described. The protein-called CNF-is active in inhibiting the duplication of cultured mammalian cells. Since nuclei division is apparently normal, treatment of cultured cells with CNF leads to the formation of gigantic, polynucleated cells. The purified protein is chromatographically and electrophoretically homogeneous. A partial characterization of CNF protein is also given.
描述了一种从大肠杆菌致病菌株中获得的蛋白质的纯化程序。这种名为CNF的蛋白质在抑制培养的哺乳动物细胞复制方面具有活性。由于细胞核分裂明显正常，用CNF处理培养细胞会导致形成巨大的多核细胞。纯化后的蛋白质在色谱和电泳方面均表现为均一。还给出了CNF蛋白的部分特性描述。
