Li J Y, Knapp R J, Sternberger L A
Cell Tissue Res. 1984;235(2):263-6. doi: 10.1007/BF00217849.
Immunocytochemistry of paraffin sections of Bouin-fixed rat pituitaries with antiserum to luteinizing-hormone-releasing hormone (LHRH) revealed two types of cells. Type I cells exhibit granular staining throughout their cytoplasm. The immunoreactivity of type II cells is confined to a much smaller area of the cytoplasm. Type I cells are located in the ventral margin of the pars intermedia, the region between the pars intermedia and the pars distalis, and the pars distalis adjacent to this region. Type II cells have a broader distribution in the pars distalis, but tend to concentrate in the region of the pars distalis near the pars intermedia. Type I cells are distinct from gonadotropes. Type II cells appear to comprise a subgroup of gonadotropes. Staining in type I, but not type II, cells in pituitary explants, maintained in serum-free media for seven days, is as intense as that in normal pituitary tissue. The data suggest that the type I cells are producing an intrinsic LHRH-like material and may be responsible, in part, for the regulation of some gonadotropes.
用抗促黄体生成激素释放激素（LHRH）抗血清对经Bouin固定的大鼠垂体石蜡切片进行免疫细胞化学分析，发现了两种类型的细胞。I型细胞在整个细胞质中呈现颗粒状染色。II型细胞的免疫反应局限于细胞质中一个小得多的区域。I型细胞位于中间部的腹侧边缘、中间部与远侧部之间的区域以及与该区域相邻的远侧部。II型细胞在远侧部分布更广，但倾向于集中在远侧部靠近中间部的区域。I型细胞与促性腺激素细胞不同。II型细胞似乎构成促性腺激素细胞的一个亚群。在无血清培养基中培养7天的垂体外植体中，I型细胞（而非II型细胞）的染色与正常垂体组织中的染色一样强烈。数据表明，I型细胞正在产生一种内源性LHRH样物质，并且可能部分负责某些促性腺激素细胞的调节。
