Baker B L, Dermody W C, Reel J R
Endocrinology. 1975 Jul;97(1):125-35. doi: 10.1210/endo-97-1-125.
The distribution of gonadotropin-releasing hormone (GnRH) was studied in the brain of adult female rats with three immunocytochemical techniques using antisera to unconjugated synthetic GnRH and to GnRH conjugated with limpet hemocyanin. GnRH was found in nervous tissue surrounding blood vessels of the organum vasculosum of the lamina terminalis. In the median eminence it occurred in nervous tissue associated primarily with the tuberoinfundibular sulci throughout their extent. Cephalic to the pars tuberalis GnRH often spread across the median eminence from sulcus to sulcus. Caudally, with widening of the median eminence, GnRH occurred dorsal to the tuberoinfundibular sulci, and especially in the external lamina medial to the sulci. A broad median zone of the median eminence was rather free of GnRH. GnRH was most concentrated in the region of continuity between the dorsolateral walls of the infundibulum and floor of the third ventricle where the tuberoinfundibular sulci are deep. Caudal to the infundibulum GnRH was disposed in a flat zone through the cephalic portion of the floor of the mammillary recess. In the median eminence GnRH appeared to be located in axons that terminated there. The amount of demonstrable GnRH varied significantly from rat to rat. The distributions of GnRH as revealed by use of antisera to unconjugated and conjugated GnRH were essentially the same. The apparent order of sensitivity of the immunocytochemical methods was: the peroxidase-antiperoxidase (PAP) (Sternberger et al.) procedure greater than the immunoglobulin-enzyme bridge (Mason et al.) procedure smaller than the conjugated antibody (Nakane and Pierce) procedure.
采用针对未结合的合成促性腺激素释放激素（GnRH）以及与帽贝血蓝蛋白结合的GnRH的抗血清，运用三种免疫细胞化学技术，对成年雌性大鼠脑内GnRH的分布进行了研究。在终板血管器血管周围的神经组织中发现了GnRH。在正中隆起中，GnRH主要出现在与贯穿其全长的结节漏斗沟相关的神经组织中。在结节部头部，GnRH常从一个沟扩散至另一个沟，横跨正中隆起。在尾部，随着正中隆起变宽，GnRH出现在结节漏斗沟的背侧，尤其是在沟内侧的外层。正中隆起的一个宽阔的中间区域GnRH含量较少。GnRH最集中于漏斗背外侧壁与第三脑室底部的连续区域，此处结节漏斗沟较深。在漏斗尾部，GnRH分布于乳头体隐窝底部头部的一个平坦区域。在正中隆起中，GnRH似乎位于终止于此的轴突中。不同大鼠之间可检测到的GnRH量差异显著。使用针对未结合和结合GnRH的抗血清所显示的GnRH分布基本相同。免疫细胞化学方法的明显敏感性顺序为：过氧化物酶 - 抗过氧化物酶（PAP）（斯特恩伯格等人）法＞免疫球蛋白 - 酶桥（梅森等人）法＜结合抗体（中根和皮尔斯）法。
