酿酒酵母中包被小泡的鉴定。
Identification of coated vesicles in Saccharomyces cerevisiae.
作者信息
Mueller S C, Branton D
出版信息
J Cell Biol. 1984 Jan;98(1):341-6. doi: 10.1083/jcb.98.1.341.
Abstract
Clathrin-coated vesicles were found in yeast, Saccharomyces cerevisiae, and enriched from spheroplasts by a rapid procedure utilizing gel filtration on Sephacryl S-1000. The coated vesicles (62-nm diam) were visualized by negative stain electron microscopy and clathrin triskelions were observed by rotary shadowing. The contour length of a triskelion leg was 490 nm. Coated vesicle fractions contain a prominent band with molecular weight of approximately 185,000 when analyzed by SDS PAGE. The presence of coated vesicles in yeast cells suggests that this organism will be useful for studying the function of clathrin-coated vesicles.
摘要
在酿酒酵母中发现了网格蛋白包被囊泡，通过利用Sephacryl S - 1000凝胶过滤的快速程序从原生质球中富集得到。通过负染电子显微镜观察到包被囊泡（直径62纳米），通过旋转阴影法观察到网格蛋白三脚复合体。三脚复合体腿的轮廓长度为490纳米。当通过SDS - PAGE分析时，包被囊泡组分含有一条分子量约为185,000的显著条带。酵母细胞中存在包被囊泡表明该生物体将有助于研究网格蛋白包被囊泡的功能。
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