Brown J K, Frey M J, Reed B R, Leff A R, Shields R, Gold W M
J Allergy Clin Immunol. 1984 Apr;73(4):473-8. doi: 10.1016/0091-6749(84)90357-9.
Because adherence of histamine to glass is well-known, we tested for its adherence to a mechanical homogenizer commonly used in the extraction of histamine from tissue samples. During 60 sec of homogenization, 15% to 17% of the histamine originally present in the samples "disappeared," and the reason for the disappearance was reversible binding of histamine to the homogenizer. Adding trace amounts of [14C]histamine to each sample before homogenization and measuring the disappearance of radioactivity during homogenization permitted correction for binding to the homogenizer. This technique for correction was validated by the measurement of endogenous concentrations of histamine in the tracheal posterior membranes of six dogs (range of mean concentrations: 0.63 to 1.51 ng/mg wet weight) followed by the measurement of known amounts of exogenous histamine added before homogenization to tracheal tissue samples from the same dogs. In the latter samples, 96 +/- 13% (mean +/- SEM) of the histamine added was measured by our technique. We conclude that binding of histamine to mechanical homogenizers may be an important cause of inaccuracy of the enzymatic assay for the measurement of histamine concentrations in tissue but that such binding may but that such binding may be easily corrected for.
由于组胺与玻璃的结合是众所周知的，我们测试了其与常用于从组织样本中提取组胺的机械匀浆器的结合情况。在匀浆60秒期间，样本中最初存在的组胺有15%至17%“消失”了，消失的原因是组胺与匀浆器的可逆结合。在匀浆前向每个样本中添加微量的[14C]组胺，并测量匀浆过程中放射性的消失情况，从而可以校正与匀浆器的结合。通过测量6只狗气管后膜中组胺的内源性浓度（平均浓度范围：0.63至1.51 ng/mg湿重），然后测量在匀浆前向来自同一只狗的气管组织样本中添加的已知量的外源性组胺，验证了这种校正技术。在后者的样本中，我们的技术测量到添加的组胺中有96±13%（平均值±标准误）。我们得出结论，组胺与机械匀浆器的结合可能是组织中组胺浓度酶法测定不准确的一个重要原因，但这种结合可能很容易校正。
