Erger R A, Casale T B
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
J Immunol Methods. 1992 Jul 31;152(1):115-21. doi: 10.1016/0022-1759(92)90094-a.
The measurement of histamine in samples obtained from human lung is important in determining the roles of histamine and mast cells in normal and disease states. We, therefore, compared different assays for the measurement of histamine in human lung samples. Both a single isotope enzymatic assay and a radioimmunoassay (RIA) were capable of accurately measuring the low concentrations of histamine (0.05-2.0 ng/ml) normally found in bronchoalveolar lavage fluid. The RIA was also able to measure histamine levels up to 1500 ng/ml in human lung tissue samples. Moreover, the RIA measurement of high levels of histamine in lung samples compared favorably to an automated spectrofluorometric method. Unlike either the single isotope enzymatic assay or the automated spectrofluorometric assay which have effective capabilities at less than and greater than 2 ng/ml, respectively, the RIA can accurately measure histamine levels from 0.05 to 1500 ng/ml. Since the RIA is easier to perform, less costly, and has a wider range of effectiveness, this assay should prove valuable in assessing histamine levels from a variety of human lung samples, thereby, providing an avenue to elucidate the roles of histamine and mast cells in lung functions.
测定从人肺获取的样本中的组胺，对于确定组胺和肥大细胞在正常及疾病状态下的作用至关重要。因此，我们比较了不同的人肺样本组胺测定方法。单同位素酶法和放射免疫分析法（RIA）都能够准确测量支气管肺泡灌洗液中通常存在的低浓度组胺（0.05 - 2.0 ng/ml）。RIA还能够测量人肺组织样本中高达1500 ng/ml的组胺水平。此外，RIA对肺样本中高水平组胺的测量结果与自动荧光分光光度法相比具有优势。与单同位素酶法和自动荧光分光光度法不同，前者分别在低于和高于2 ng/ml时具有有效能力，而RIA能够准确测量0.05至1500 ng/ml的组胺水平。由于RIA操作更简便、成本更低且有效性范围更广，该测定方法在评估各种人肺样本中的组胺水平时应具有重要价值，从而为阐明组胺和肥大细胞在肺功能中的作用提供一条途径。
