van Landeghem A A, Poortman J, Helmond-Agema A, Thijssen J H
J Steroid Biochem. 1984 Feb;20(2):639-44. doi: 10.1016/0022-4731(84)90136-5.
A reliable method for the extraction of steroid hormones from human uterine tissue and the subsequent measurement of these hormones in the subcellular compartments by radioimmunoassay is described. Extraction of radioactive steroid hormones from in vivo labelled human uterine tissue by different methods reveals that an almost quantitative extraction of steroid hormones from the nuclear fraction is obtained by sonication in ethanol-acetone. Extraction of steroid hormones with diethylether from a high speed cytosol is incomplete. Using a more potent denaturating agent prior to extraction with diethyl ether leads to complete extraction of unconjugated steroids.
本文描述了一种从人子宫组织中提取甾体激素并随后通过放射免疫分析法在亚细胞区室中测量这些激素的可靠方法。通过不同方法从体内标记的人子宫组织中提取放射性甾体激素表明，通过在乙醇 - 丙酮中超声处理可从核部分几乎定量地提取甾体激素。用乙醚从高速胞质溶胶中提取甾体激素是不完全的。在用乙醚提取之前使用更强效的变性剂可导致未结合甾体的完全提取。
